Abstract
Detection of foodborne microorganisms is an imperative component of a food safety plan for meat processors. Detection of pathogenic bacteria constitutes a tremendous challenge therefore, traditional detection methods include a rigorous multistep process, such as enrichment, isolation, and further biochemical/serological identification. Consequently, different DNA-based molecular detection techniques have been developed in order to reduce time needed to make a decision to accept or reject product and increase sensitivity/specificity of detection. Additionally, DNA sequence-based subtyping methods have improved the discriminatory power and epidemiological concordance of characterizing bacterial foodborne pathogens involved in outbreak investigations. In this article, the most current and commonly employed DNA-based detection and subtyping methods are reviewed.
Original language | English |
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Title of host publication | Encyclopedia of Meat Sciences |
Publisher | Elsevier Inc. |
Pages | 294-300 |
Number of pages | 7 |
ISBN (Electronic) | 9780123847317 |
ISBN (Print) | 9780123847348 |
DOIs | |
State | Published - Jan 1 2014 |
Keywords
- Bacteria
- DNA-based methods
- Detection
- Epidemiology
- Food safety
- Foodborne pathogens
- Isothermal detection
- Meatborne pathogens
- Microorganisms
- Molecular methods
- Molecular serotyping
- PCR
- PFGE
- Subtyping methods