DNA detection using laser transmission spectroscopy

F. Li, A. R. Mahon, M. A. Barnes, S. Egan, J. Feder, D. M. Lodge, C. T. Hwang, R. Schafer, S. T. Ruggiero, C. E. Tanner

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Laser transmission spectroscopy (LTS) [1] is a new quantitative and rapid technique for measuring the size, shape, and number of nanoparticles in suspension. We report on the application of LTS as a novel detection method for species-specific DNA where the presence of one invasive species was differentiated from a closely related invasive sister species. The method employs carboxylated polystyrene nanoparticles functionalized with short DNA fragments that are complimentary to a specific target DNA sequence. In solution, the DNA strands containing targets bind to the tags resulting in a sizable increase in the nanoparticle diameter, which is rapidly and quantitatively measured using LTS. DNA strands that do not contain the target sequence do not bind and produce no size change of the carboxylated beads. The results show that LTS has the potential to become a quantitative and rapid DNA detection method and have additional applications for point-of-care medical diagnostics.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012
Pages48-51
Number of pages4
StatePublished - 2012
EventNanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012 - Santa Clara, CA, United States
Duration: Jun 18 2012Jun 21 2012

Publication series

NameTechnical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012

Conference

ConferenceNanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012
CountryUnited States
CitySanta Clara, CA
Period06/18/1206/21/12

Keywords

  • DNA detection
  • Dynamic light scattering
  • Invasive species
  • Laser transmission spectroscopy
  • Nanoparticle
  • Particle size distribution

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