TY - JOUR
T1 - Distribution and development of molecularly distinct perineuronal nets in visual thalamus
AU - Zabet Moghaddam, Masoud
AU - sabbagh, u
AU - su, k
AU - Monavarfeshani, Aboozar
AU - Cole, James
AU - Carnival, Eric
AU - Su, Jianmin
AU - Mirzaei, Mehdi
AU - Gupta, Vivek
AU - Salekdeh, Ghasem Hosseini
AU - Fox, Michael A.
N1 - Funding Information:
This work was supported in part by the National Institutes of Health (EY021222 and EY024712 [MAF]), an Independent Investigator grant from the Brain and Behavior Foundation (MAF), and a fellowship from the VTCRI Medical Research Scholars Program (AM). All authors declare no actual or potential conflicts of interest.
Publisher Copyright:
© 2018 International Society for Neurochemistry
PY - 2018/12
Y1 - 2018/12
N2 - Visual information is detected by the retina and transmitted into the brain by retinal ganglion cells. In rodents, the visual thalamus is a major recipient of retinal ganglion cells axons and is divided into three functionally distinct nuclei: the dorsal lateral geniculate nucleus (dLGN), ventral LGN (vLGN), and intergeniculate leaflet. Despite being densely innervated by retinal input, each nucleus in rodent visual thalamus possesses diverse molecular profiles which underpin their unique circuitry and cytoarchitecture. Here, we combined large-scale unbiased proteomic and transcriptomic analyses to elucidate the molecular expression profiles of the developing mouse dLGN and vLGN. We identified several extracellular matrix proteins as differentially expressed in these regions, particularly constituent molecules of perineuronal nets (PNNs). Remarkably, we discovered at least two types of molecularly distinct Aggrecan-rich PNN populations in vLGN, exhibiting non-overlapping spatial, temporal, and cell-type specific expression patterns. The mechanisms responsible for the formation of these two populations of PNNs also differ as the formation of Cat315+ PNNs (but not WFA+ PNNs) required input from the retina. This study is first to suggest that cell type- and molecularly specific supramolecular assemblies of extracellular matrix may play important roles in the circuitry associated with the subcortical visual system and in the processing of visual information. Open science badges: This article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form for this article can be found at the end of the article. More information about the Open Practices badges can be found at https://cos.io/our-services/open-science-badges/. (Figure presented.). Cover Image for this issue: doi: 10.1111/jnc.14203.
AB - Visual information is detected by the retina and transmitted into the brain by retinal ganglion cells. In rodents, the visual thalamus is a major recipient of retinal ganglion cells axons and is divided into three functionally distinct nuclei: the dorsal lateral geniculate nucleus (dLGN), ventral LGN (vLGN), and intergeniculate leaflet. Despite being densely innervated by retinal input, each nucleus in rodent visual thalamus possesses diverse molecular profiles which underpin their unique circuitry and cytoarchitecture. Here, we combined large-scale unbiased proteomic and transcriptomic analyses to elucidate the molecular expression profiles of the developing mouse dLGN and vLGN. We identified several extracellular matrix proteins as differentially expressed in these regions, particularly constituent molecules of perineuronal nets (PNNs). Remarkably, we discovered at least two types of molecularly distinct Aggrecan-rich PNN populations in vLGN, exhibiting non-overlapping spatial, temporal, and cell-type specific expression patterns. The mechanisms responsible for the formation of these two populations of PNNs also differ as the formation of Cat315+ PNNs (but not WFA+ PNNs) required input from the retina. This study is first to suggest that cell type- and molecularly specific supramolecular assemblies of extracellular matrix may play important roles in the circuitry associated with the subcortical visual system and in the processing of visual information. Open science badges: This article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form for this article can be found at the end of the article. More information about the Open Practices badges can be found at https://cos.io/our-services/open-science-badges/. (Figure presented.). Cover Image for this issue: doi: 10.1111/jnc.14203.
KW - LGN
KW - aggrecan
KW - perineuronal net
KW - retinogeniculate
KW - thalamus
KW - visual
UR - http://www.scopus.com/inward/record.url?scp=85057259726&partnerID=8YFLogxK
U2 - 10.1111/jnc.14614
DO - 10.1111/jnc.14614
M3 - Article
C2 - 30326149
VL - 147
SP - 626
EP - 646
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 5
ER -