Direct quantification of AD-36 adenovirus DNA by capillary electrophoresis with laser-induced fluorescence

Jill M. Kolesar, Judith A. Miller, Nikhil V. Dhurandhar, Richard L. Atkinson

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

An adenovirus, AD-36, has been linked to human adiposity and a sensitive and reliable quantitative method is required to assess AD-36 viral loads. This report describes direct detection of AD-36 viral DNA, which is the first method to quantitate DNA without amplification. Total genomic DNA is hybridized with an AD-36 specific fluorescently labeled probe and analyzed by capillary electrophoresis with laser-induced fluorescence. The minimum detectable quantity is 10.3 ng/ml, corresponding to 282 copies of AD-36 with a precision of 1-6%. These results indicate that direct detection with capillary electrophoresis with laser-induced fluorescence (CE-LIF) is a reliable and sensitive method for quantifying AD-36 viral DNA. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume744
Issue number1
DOIs
StatePublished - Jul 7 2000

Keywords

  • AD-36 Adenovirus
  • DNA
  • Gene quantification

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