Differential Mobility Cytometry

Kelong Wang, Ximena Solis-Wever, Charmaine Aguas, Yan Liu, Peng Li, Dimitrios Pappas

Research output: Contribution to journalArticlepeer-review

Abstract

A new cell analysis method, Differential Mobility Cytometry (DMC), was developed to monitor cells spatially and temporally, or to separate cells based on affinity interactions. DMC combines of an oscillation system with open-tubular capillary cell affinity chromatography (OT-CAC), although any separation volume (capillaries, channels, etc) can be used. This unique separation approach uses oscillating flow and differential imaging to analyze cells as they retard and adhere to an affinity surface. Three main factors of the oscillation system were studied: the pump speed, oscillation frequency and the cell velocity at different oscillation speeds. The optimized oscillation frequency and intensity were determined. Cell-surface interactions were used to estimate the number of bonds formed during cell capture. An average of 200 bonds (standard deviation 150 bonds) were formed during cell capture. The variability was due to differences in cell capture time (0.8 ± 0.6 seconds). Cells
Original languageEnglish
Pages (from-to)3334-3343
JournalAnalytical Chemistry
StatePublished - 2009

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