TY - JOUR
T1 - Differential Mobility Cytometry
AU - Wang, Kelong
AU - Solis-Wever, Ximena
AU - Aguas, Charmaine
AU - Liu, Yan
AU - Li, Peng
AU - Pappas, Dimitrios
PY - 2009
Y1 - 2009
N2 - A new cell analysis method, Differential Mobility Cytometry (DMC), was developed to monitor cells spatially and temporally, or to separate cells based on affinity interactions. DMC combines of an oscillation system with open-tubular capillary cell affinity chromatography (OT-CAC), although any separation volume (capillaries, channels, etc) can be used. This unique separation approach uses oscillating flow and differential imaging to analyze cells as they retard and adhere to an affinity surface. Three main factors of the oscillation system were studied: the pump speed, oscillation frequency and the cell velocity at different oscillation speeds. The optimized oscillation frequency and intensity were determined. Cell-surface interactions were used to estimate the number of bonds formed during cell capture. An average of 200 bonds (standard deviation 150 bonds) were formed during cell capture. The variability was due to differences in cell capture time (0.8 ± 0.6 seconds). Cells
AB - A new cell analysis method, Differential Mobility Cytometry (DMC), was developed to monitor cells spatially and temporally, or to separate cells based on affinity interactions. DMC combines of an oscillation system with open-tubular capillary cell affinity chromatography (OT-CAC), although any separation volume (capillaries, channels, etc) can be used. This unique separation approach uses oscillating flow and differential imaging to analyze cells as they retard and adhere to an affinity surface. Three main factors of the oscillation system were studied: the pump speed, oscillation frequency and the cell velocity at different oscillation speeds. The optimized oscillation frequency and intensity were determined. Cell-surface interactions were used to estimate the number of bonds formed during cell capture. An average of 200 bonds (standard deviation 150 bonds) were formed during cell capture. The variability was due to differences in cell capture time (0.8 ± 0.6 seconds). Cells
M3 - Article
SP - 3334
EP - 3343
JO - Analytical Chemistry
JF - Analytical Chemistry
ER -