Diff-Quik® Evaluation of Round Cells and Sperm

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Diff-Quik is a proprietary brand of a Romanowski-type stain that consists of a light blue fixative (triarylmethane dye), a red stain (eosin, xanthene-type dye), and a blue stain (thiazine dye). Diff-Quik stain has been used primarily to assess round cells in a semen sample, but may also be used, with some modification in staining technique, to assess spermatozoa. Staining with a Diff-Quik stain offers an alternative to the more common eosin-nigrosin stain for the evaluation of sperm morphology. This chapter discusses the step by step procedure to assess the round cells and spermatozoa with illustrations. It outlines the necessary conditions to be followed for the Diff-Quik evaluation of round cells and sperm.

Original languageEnglish
Title of host publicationEquine Reproductive Procedures
Number of pages2
ISBN (Electronic)9781118904398
ISBN (Print)9780470960394
StatePublished - Aug 25 2014


  • Diff-Quik evaluation
  • Round cells
  • Spermatozoa


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