Diagnosing Strongylus vulgaris in pooled fecal samples,

Martin k, Chelsea B Facison, Jessica A Scare, Avery N Martin, Holli S Gravatte, Ashley Steuer

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: Strongylus vulgaris is the most pathogenic intestinal helminth parasite infecting horses. The migrating larvae in the mesenteric blood vessels can cause non-strangulating intestinal infarctions, which have a guarded prognosis for survival. Infections are typically diagnosed by coproculture, but a PCR test is available in some countries. While it is ideal to test horses individually, many veterinarians and clients wish to pool samples to reduce workload and cost of the diagnostic method. The purpose of this study was to determine if pooling of fecal samples would negatively impact diagnostic performance of the coproculture and the PCR for determination of S. vulgaris infection. Ten horses with strongylid eggs per gram (EPG) >500 and confirmed as either S. vulgaris positive or negative were selected as fecal donors. Eight pools with feces from five horses were created with 0%, 10 %, 20 %, 30 %, 40 %, 50 %, 80 %, and 100 % S. vulgaris positive feces. From each pool, 20
Original languageEnglish
Pages (from-to)4(https://www.sciencedirect.com/science/article/pii/S0304401721001539)
JournalVeterinary Parasitology
StatePublished - Aug 2021

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