Dexamethasone regulation of terminal differentiation in 3T3-F442A preadipocyte cell line

Naïma Moustaïd, Bernard Hainque, Annie Quignard-Boulangé

Research output: Contribution to journalReview articlepeer-review

10 Scopus citations

Abstract

The 3T3-F442A preadipocyte cell line was previously shown to possess specific glucocorticoid receptors whose number increased in the time course of differentiation. We have examined the effects of a three day dexamethasone treatment, added at confluence, on cells differentiated in the presence or absence of insulin. Triglyceride accumulation, polyamine content as well as glycerophosphate dehydrogenase and fatty acid synthetase activities were measured during the adipose conversion. We have also determined 2-deoxyglucose uptake in non-differentiated and differentiated cells. Dexamethasone was shown to decrease the adipose conversion by 3T3-F442A cells in the presence or absence of insulin. Intracellular spermidine content in differentiating cells was sensitive to dexamethasone and insulin in the same way as an enzymatic marker of terminal differentiation, glycerophosphate dehydrogenase. Dexamethasone decreases the 2 deoxyglucose uptake in non-differentiated and differentiated cells while insulin increases this uptake only in differentiated cells. This work shows that glucocorticoids inhibit adipocyte metabolism at distinct levels and suggests that these hormones might play an important role in the regulation of adipose tissue mass.

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalCytotechnology
Volume1
Issue number4
DOIs
StatePublished - Nov 1988

Keywords

  • 3T3-F442A cells
  • dexamethasone
  • fatty acid synthetase
  • glycerophosphate dehydrogenase
  • polyamines

Fingerprint Dive into the research topics of 'Dexamethasone regulation of terminal differentiation in 3T3-F442A preadipocyte cell line'. Together they form a unique fingerprint.

Cite this