Development of high-sensitivity ion trap ion mobility spectrometry time-of-flight techniques: A high-throughput nano-LC-IMS-TOF separation of peptides arising from a Drosophila protein extract

Sunnie Myung, Young Jin Lee, Myeong Hee Moon, John Taraszka, Rena Sowell, Stormy Koeniger, Amy E. Hilderbrand, Stephen J. Valentine, Lucy Cherbas, Peter Cherbas, Thomas C. Kaufmann, David F. Miller, Yehia Mechref, Milos V. Novotny, Michael A. Ewing, C. Ray Sporieder, David E. Clemmer

Research output: Contribution to journalArticlepeer-review

98 Scopus citations

Abstract

A linear octopole trap interface for an ion mobility time-of-flight mass spectrometer has been developed for focusing and accumulating continuous beams of ions produced by electrospray ionization. The interface improves experimental efficiencies by factors of ∼50-200 compared with an analogous configuration that utilizes a three-dimensional Paul geometry trap (Hoaglund-Hyzer, C. S.; Lee, Y. J.; Counterman, A. E.; Clemmer, D. E. Anal. Chem. 2002, 74, 992-1006). With these improvements, it is possible to record nested drift (flight) time distributions for complex mixtures in fractions of a second. We demonstrate the approach for several well-defined peptide mixtures and an assessment of the detection limits is given. Additionally, we demonstrate the utility of the approach in the field of proteomics by an on-line, three-dimensional nano-LC-ion mobility-TOF separation of tryptic peptides from the Drosophila proteome.

Original languageEnglish
Pages (from-to)5137-5145
Number of pages9
JournalAnalytical Chemistry
Volume75
Issue number19
DOIs
StatePublished - Oct 1 2003

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