TY - JOUR
T1 - Development of a transdermal salmonella challenge model in calves3
AU - Edrington, T. S.
AU - Loneragan, G. H.
AU - Hill, J.
AU - Genovese, K. J.
AU - He, H.
AU - Callaway, T. R.
AU - Anderson, R. C.
AU - Brichta-Harhay, D. M.
AU - Nisbet, D. J.
PY - 2013/7
Y1 - 2013/7
N2 - Recent investigations have found that Salmonella can be routinely recovered from peripheral lymph nodes (PLNs) of cattle presented for harvest. When contained within the PLNs, this foodborne pathogen is protected from currently used postharvest, inplant intervention strategies and, therefore, PLNs harboring Salmonella may be a potential contaminant of ground beef. The objective of this work was to develop a challenge model that effectively and repeatedly results in Salmonella-positive PLNs. A 10-lancet skin-allergy instrument was inoculated with Salmonella, and calves were inoculated intra- and/or transdermally by applying the device over various ventral regions of the skin. Salmonella was successfully and predictably recovered from regionspecific PLNs up to 8 days postchallenge. Furthermore, serotypes inoculated within specific regions were only recovered from the PLNs draining those regions. This model provides a method to predictably infect PLNs with Salmonella. Further, this model makes it possible to determine the duration of infection and to evaluate candidate interventions that may shorten the duration of infection.
AB - Recent investigations have found that Salmonella can be routinely recovered from peripheral lymph nodes (PLNs) of cattle presented for harvest. When contained within the PLNs, this foodborne pathogen is protected from currently used postharvest, inplant intervention strategies and, therefore, PLNs harboring Salmonella may be a potential contaminant of ground beef. The objective of this work was to develop a challenge model that effectively and repeatedly results in Salmonella-positive PLNs. A 10-lancet skin-allergy instrument was inoculated with Salmonella, and calves were inoculated intra- and/or transdermally by applying the device over various ventral regions of the skin. Salmonella was successfully and predictably recovered from regionspecific PLNs up to 8 days postchallenge. Furthermore, serotypes inoculated within specific regions were only recovered from the PLNs draining those regions. This model provides a method to predictably infect PLNs with Salmonella. Further, this model makes it possible to determine the duration of infection and to evaluate candidate interventions that may shorten the duration of infection.
UR - http://www.scopus.com/inward/record.url?scp=84880281476&partnerID=8YFLogxK
U2 - 10.4315/0362-028X.JFP-12-317
DO - 10.4315/0362-028X.JFP-12-317
M3 - Article
C2 - 23834802
AN - SCOPUS:84880281476
SN - 0362-028X
VL - 76
SP - 1255
EP - 1258
JO - Journal of food protection
JF - Journal of food protection
IS - 7
ER -