Determination of salsolinol and related catecholamines through on-line preconcentration and liquid chromatography/atmospheric pressure photoionization mass spectrometry

Jason A. Starkey; Yehia Mechref; Jan Muzikar; William J. McBride; Milos V. Novotny

doi:10.1021/ac051863j

Determination of salsolinol and related catecholamines through on-line preconcentration and liquid chromatography/atmospheric pressure photoionization mass spectrometry

Jason A. Starkey, Yehia Mechref, Jan Muzikar, William J. McBride, Milos V. Novotny

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A new analytical approach has been developed for simultaneous measurements of endogenous salsolinol and major catecholamines in brain tissue of experimental animals. This procedure involves a combination of on-line phenyl boronate affinity preconcentration and microcolumn liquid chromatography, followed by mass spectrometry equipped with an atmospheric pressure photoionization (APPI) source. Flow conditions of the APPI source were optimized for detection sensitivity while different dopants were evaluated. The on-line preconcentration was found essential for the sensitivity requirements of salsolinol measurements in the brain tissue from alcohol-preferring rats subjected to different levels of alcohol exposure.

Original language	English
Pages (from-to)	3342-3347
Number of pages	6
Journal	Analytical Chemistry
Volume	78
Issue number	10
DOIs	https://doi.org/10.1021/ac051863j
State	Published - May 15 2006

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10.1021/ac051863j

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abstract = "A new analytical approach has been developed for simultaneous measurements of endogenous salsolinol and major catecholamines in brain tissue of experimental animals. This procedure involves a combination of on-line phenyl boronate affinity preconcentration and microcolumn liquid chromatography, followed by mass spectrometry equipped with an atmospheric pressure photoionization (APPI) source. Flow conditions of the APPI source were optimized for detection sensitivity while different dopants were evaluated. The on-line preconcentration was found essential for the sensitivity requirements of salsolinol measurements in the brain tissue from alcohol-preferring rats subjected to different levels of alcohol exposure.",

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AU - Starkey, Jason A.

AU - Mechref, Yehia

AU - Muzikar, Jan

AU - McBride, William J.

AU - Novotny, Milos V.

PY - 2006/5/15

Y1 - 2006/5/15

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AB - A new analytical approach has been developed for simultaneous measurements of endogenous salsolinol and major catecholamines in brain tissue of experimental animals. This procedure involves a combination of on-line phenyl boronate affinity preconcentration and microcolumn liquid chromatography, followed by mass spectrometry equipped with an atmospheric pressure photoionization (APPI) source. Flow conditions of the APPI source were optimized for detection sensitivity while different dopants were evaluated. The on-line preconcentration was found essential for the sensitivity requirements of salsolinol measurements in the brain tissue from alcohol-preferring rats subjected to different levels of alcohol exposure.

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JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 10

ER -

Determination of salsolinol and related catecholamines through on-line preconcentration and liquid chromatography/atmospheric pressure photoionization mass spectrometry

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