Detection of the amphibian pathogens chytrid fungus (Batrachochytrium dendrobatidis) and ranavirus in West Texas, USA, using environmental DNA

Matthew A. Barnes; Audrey D. Brown; Mikaela N. Daum; Karla A. de la Garza; Jackson Driskill; Kylie Garrett; Madeleine S. Goldstein; Alaia Luk; Joel I. Maguire; Robert Moke; Emily M. Ostermaier; Yorick M. Sanders; Theodore Sandhu; Aryiah Stith; Varshini V. Suresh

doi:10.7589/2019-08-212

Detection of the amphibian pathogens chytrid fungus (Batrachochytrium dendrobatidis) and ranavirus in West Texas, USA, using environmental DNA

Matthew A. Barnes, Audrey D. Brown, Mikaela N. Daum, Karla A. de la Garza, Jackson Driskill, Kylie Garrett, Madeleine S. Goldstein, Alaia Luk, Joel I. Maguire, Robert Moke, Emily M. Ostermaier, Yorick M. Sanders, Theodore Sandhu, Aryiah Stith, Varshini V. Suresh

Natural Resources Management

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10 Scopus citations

Abstract

Environmental DNA (eDNA) meth ods provide novel options for the detection of pathogens. The amphibian pathogens Batrachochytrium dendrobatidis (Bd) and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River basin near the Texas panhandle. We collected water samples from five urban playa lakes and one reservoir in and around Lubbock, Texas. Quantitative PCR detected both Bd and Ranavirus at one playa lake, representing novel detection of both pathogens in the region. Based on these results, we recommend increased monitoring for the pathogens and symptoms of amphibian disease throughout the region.

Original language	English
Pages (from-to)	702-706
Number of pages	5
Journal	Journal of wildlife diseases
Volume	56
Issue number	3
DOIs	https://doi.org/10.7589/2019-08-212
State	Published - Jul 2020

Keywords

Amphibian disease
Bd
Chytrid fungus
EDNA
Ranavirus
West Texas

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Barnes, M. A., Brown, A. D., Daum, M. N., de la Garza, K. A., Driskill, J., Garrett, K., Goldstein, M. S., Luk, A., Maguire, J. I., Moke, R., Ostermaier, E. M., Sanders, Y. M., Sandhu, T., Stith, A., & Suresh, V. V. (2020). Detection of the amphibian pathogens chytrid fungus (Batrachochytrium dendrobatidis) and ranavirus in West Texas, USA, using environmental DNA. Journal of wildlife diseases, 56(3), 702-706. https://doi.org/10.7589/2019-08-212

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title = "Detection of the amphibian pathogens chytrid fungus (Batrachochytrium dendrobatidis) and ranavirus in West Texas, USA, using environmental DNA",

abstract = "Environmental DNA (eDNA) meth ods provide novel options for the detection of pathogens. The amphibian pathogens Batrachochytrium dendrobatidis (Bd) and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River basin near the Texas panhandle. We collected water samples from five urban playa lakes and one reservoir in and around Lubbock, Texas. Quantitative PCR detected both Bd and Ranavirus at one playa lake, representing novel detection of both pathogens in the region. Based on these results, we recommend increased monitoring for the pathogens and symptoms of amphibian disease throughout the region.",

keywords = "Amphibian disease, Bd, Chytrid fungus, EDNA, Ranavirus, West Texas",

author = "Barnes, {Matthew A.} and Brown, {Audrey D.} and Daum, {Mikaela N.} and {de la Garza}, {Karla A.} and Jackson Driskill and Kylie Garrett and Goldstein, {Madeleine S.} and Alaia Luk and Maguire, {Joel I.} and Robert Moke and Ostermaier, {Emily M.} and Sanders, {Yorick M.} and Theodore Sandhu and Aryiah Stith and Suresh, {Varshini V.}",

note = "Publisher Copyright: {\textcopyright} Wildlife Disease Association 2020.",

year = "2020",

month = jul,

doi = "10.7589/2019-08-212",

language = "English",

volume = "56",

pages = "702--706",

journal = "Journal of wildlife diseases",

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Barnes, MA, Brown, AD, Daum, MN, de la Garza, KA, Driskill, J, Garrett, K, Goldstein, MS, Luk, A, Maguire, JI, Moke, R, Ostermaier, EM, Sanders, YM, Sandhu, T, Stith, A & Suresh, VV 2020, 'Detection of the amphibian pathogens chytrid fungus (Batrachochytrium dendrobatidis) and ranavirus in West Texas, USA, using environmental DNA', Journal of wildlife diseases, vol. 56, no. 3, pp. 702-706. https://doi.org/10.7589/2019-08-212

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T1 - Detection of the amphibian pathogens chytrid fungus (Batrachochytrium dendrobatidis) and ranavirus in West Texas, USA, using environmental DNA

AU - Barnes, Matthew A.

AU - Brown, Audrey D.

AU - Daum, Mikaela N.

AU - de la Garza, Karla A.

AU - Driskill, Jackson

AU - Garrett, Kylie

AU - Goldstein, Madeleine S.

AU - Luk, Alaia

AU - Maguire, Joel I.

AU - Moke, Robert

AU - Ostermaier, Emily M.

AU - Sanders, Yorick M.

AU - Sandhu, Theodore

AU - Stith, Aryiah

AU - Suresh, Varshini V.

N1 - Publisher Copyright: © Wildlife Disease Association 2020.

PY - 2020/7

Y1 - 2020/7

N2 - Environmental DNA (eDNA) meth ods provide novel options for the detection of pathogens. The amphibian pathogens Batrachochytrium dendrobatidis (Bd) and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River basin near the Texas panhandle. We collected water samples from five urban playa lakes and one reservoir in and around Lubbock, Texas. Quantitative PCR detected both Bd and Ranavirus at one playa lake, representing novel detection of both pathogens in the region. Based on these results, we recommend increased monitoring for the pathogens and symptoms of amphibian disease throughout the region.

AB - Environmental DNA (eDNA) meth ods provide novel options for the detection of pathogens. The amphibian pathogens Batrachochytrium dendrobatidis (Bd) and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River basin near the Texas panhandle. We collected water samples from five urban playa lakes and one reservoir in and around Lubbock, Texas. Quantitative PCR detected both Bd and Ranavirus at one playa lake, representing novel detection of both pathogens in the region. Based on these results, we recommend increased monitoring for the pathogens and symptoms of amphibian disease throughout the region.

KW - Amphibian disease

KW - Bd

KW - Chytrid fungus

KW - EDNA

KW - Ranavirus

KW - West Texas

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SN - 0090-3558

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ER -

Detection of the amphibian pathogens chytrid fungus (Batrachochytrium dendrobatidis) and ranavirus in West Texas, USA, using environmental DNA

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Keywords

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