TY - JOUR
T1 - Defining glycoprotein cancer biomarkers by MS in conjunction with glycoprotein enrichment
AU - Song, Ehwang
AU - Mechref, Yehia
N1 - Publisher Copyright:
© 2015 Future Medicine Ltd.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Protein glycosylation is an important and common post-translational modification. More than 50% of human proteins are believed to be glycosylated to modulate the functionality of proteins. Aberrant glycosylation has been correlated to several diseases, such as inflammatory skin diseases, diabetes mellitus, cardiovascular disorders, rheumatoid arthritis, Alzheimer's and prion diseases, and cancer. Many approved cancer biomarkers are glycoproteins which are not highly abundant proteins. Therefore, effective qualitative and quantitative assessment of glycoproteins entails enrichment methods. This chapter summarizes glycoprotein enrichment methods, including lectin affinity, immunoaffinity, hydrazide chemistry, hydrophilic interaction liquid chromatography, and click chemistry. The use of these enrichment approaches in assessing the qualitative and quantitative changes of glycoproteins in different types of cancers are presented and discussed. This chapter highlights the importance of glycoprotein enrichment techniques for the identification and characterization of new reliable cancer biomarkers.
AB - Protein glycosylation is an important and common post-translational modification. More than 50% of human proteins are believed to be glycosylated to modulate the functionality of proteins. Aberrant glycosylation has been correlated to several diseases, such as inflammatory skin diseases, diabetes mellitus, cardiovascular disorders, rheumatoid arthritis, Alzheimer's and prion diseases, and cancer. Many approved cancer biomarkers are glycoproteins which are not highly abundant proteins. Therefore, effective qualitative and quantitative assessment of glycoproteins entails enrichment methods. This chapter summarizes glycoprotein enrichment methods, including lectin affinity, immunoaffinity, hydrazide chemistry, hydrophilic interaction liquid chromatography, and click chemistry. The use of these enrichment approaches in assessing the qualitative and quantitative changes of glycoproteins in different types of cancers are presented and discussed. This chapter highlights the importance of glycoprotein enrichment techniques for the identification and characterization of new reliable cancer biomarkers.
KW - HILIC enrichment
KW - LC-MS/MS
KW - cancer biomarkers
KW - click-chemistry enrichment
KW - glycoprotein enrichment
KW - glycoproteomics
KW - hydrazide chemistry
KW - immunoaffinity-based enrichment
KW - lectin chromatography
UR - http://www.scopus.com/inward/record.url?scp=84942885682&partnerID=8YFLogxK
U2 - 10.2217/bmm.15.55
DO - 10.2217/bmm.15.55
M3 - Review article
C2 - 26330015
AN - SCOPUS:84942885682
SN - 1752-0363
VL - 9
SP - 835
EP - 844
JO - Biomarkers in Medicine
JF - Biomarkers in Medicine
IS - 9
ER -