Daidzein and the daidzein metabolite, equol, enhance adipocyte differentiation and PPARγ transcriptional activity

Kae Won Cho, Ok Hwan Lee, William J. Banz, Naima Moustaid-Moussa, Neil F. Shay, Young Cheul Kim

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

Dietary soy isoflavones have been shown to favorably alter the metabolic phenotypes associated with Type 2 diabetes. However, the identification of direct targets and the underlying molecular mechanisms by which soy isoflaovones exert antidiabetic effects remain elusive. Since the insulin-sensitizing effects of thiazolidinediones, antidiabetic drugs, are mediated through activation of peroxisome proliferators-activated receptor gamma (PPARγ), we examined the effects of daidzein and the daidzein metabolite, equol, on adipocyte differentiation and PPARγ activation. In 3T3-L1 cells, daidzein enhanced adipocyte differentiation and PPARγ expression in a dose-dependent manner. Daidzein also dose-dependently increased insulin-stimulated glucose uptake and the relative abundance of insulin-responsive glucose transporter 4 (GLUT4) and insulin receptor substrate 1 (IRS-1) mRNA. In C3H10T1/2 cells, both daidzein and equol at 1 μmol/L and higher significantly increased adipocyte differentiation and insulin-stimulated glucose uptake. Furthermore, daidzein and equol up-regulated PPARγ-mediated transcriptional activity, and daidzein restored the PPARγ antagonist-induced inhibition of aP2 and GLUT4 mRNA levels. Our results indicate that daidzein enhances insulin-stimulated glucose uptake in adipocytes by increasing the expression of GLUT4 and IRS-1 via the activation of PPARγ. These data further support the recent findings that favorable effects of dietary soy isoflavones may be attributable to daidzein and its metabolite equol.

Original languageEnglish
Pages (from-to)841-847
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume21
Issue number9
DOIs
StatePublished - Sep 2010

Keywords

  • Adipocyte differentiation
  • Daidzein
  • Equol
  • GLUT4
  • Insulin sensitivity
  • PPARγ

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