Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells

Mohamed Elamir Hegazy; Sara Abdelfatah; Ahmed R. Hamed; Tarik A. Mohamed; Abdelsamed A. Elshamy; Ibrahim A. Saleh; Eman H. Reda; Nahla S. Abdel-Azim; Khaled A. Shams; Mahmoud Sakr; Yoshikazu Sugimoto; Paul Pare; Thomas Efferth

Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells

Mohamed Elamir Hegazy, Sara Abdelfatah, Ahmed R. Hamed, Tarik A. Mohamed, Abdelsamed A. Elshamy, Ibrahim A. Saleh, Eman H. Reda, Nahla S. Abdel-Azim, Khaled A. Shams, Mahmoud Sakr, Yoshikazu Sugimoto, Paul Pare, Thomas Efferth

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Background: The multidrug resistance (MDR) phenotype encounters a major challenge to the success of established chemotherapy in cancer patients. We hypothesized that cytotoxic medicinal plants with novel phytochemicals can overcome MDR and kill MDR-cells with similar efficacy as drug sensitive cells. Purpose: We evaluated plant extracts from an unexplored ecosystem in Egypt with unusual climate and nutrient conditions for their activity against sensitive and multidrug-resistant cancer cell lines. Material and methods/study design: Methylene chloride: methanol (1:1) and methanol: H2O (7:3) extracts of 40 plants were prepared resulting in a sum of 76 fraction containing compounds with varying polarity. The resazurin reduction assay was employed to evaluate the cytotoxicity of these extracts on five matched pairs of drug-sensitive and their drug-resistant cell lines. Flow cytometry and Western blotting was used …

Original language	English
Journal	Default journal
State	Published - Jun 1 2019

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title = "Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells",

abstract = "Background: The multidrug resistance (MDR) phenotype encounters a major challenge to the success of established chemotherapy in cancer patients. We hypothesized that cytotoxic medicinal plants with novel phytochemicals can overcome MDR and kill MDR-cells with similar efficacy as drug sensitive cells. Purpose: We evaluated plant extracts from an unexplored ecosystem in Egypt with unusual climate and nutrient conditions for their activity against sensitive and multidrug-resistant cancer cell lines. Material and methods/study design: Methylene chloride: methanol (1:1) and methanol: H2O (7:3) extracts of 40 plants were prepared resulting in a sum of 76 fraction containing compounds with varying polarity. The resazurin reduction assay was employed to evaluate the cytotoxicity of these extracts on five matched pairs of drug-sensitive and their drug-resistant cell lines. Flow cytometry and Western blotting was used …",

author = "Hegazy, {Mohamed Elamir} and Sara Abdelfatah and Hamed, {Ahmed R.} and Mohamed, {Tarik A.} and Elshamy, {Abdelsamed A.} and Saleh, {Ibrahim A.} and Reda, {Eman H.} and Abdel-Azim, {Nahla S.} and Shams, {Khaled A.} and Mahmoud Sakr and Yoshikazu Sugimoto and Paul Pare and Thomas Efferth",

year = "2019",

month = jun,

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language = "English",

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publisher = "Current Opinion in Endocrinology Diabetes and Obesity",

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T1 - Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells

AU - Hegazy, Mohamed Elamir

AU - Abdelfatah, Sara

AU - Hamed, Ahmed R.

AU - Mohamed, Tarik A.

AU - Elshamy, Abdelsamed A.

AU - Saleh, Ibrahim A.

AU - Reda, Eman H.

AU - Abdel-Azim, Nahla S.

AU - Shams, Khaled A.

AU - Sakr, Mahmoud

AU - Sugimoto, Yoshikazu

AU - Pare, Paul

AU - Efferth, Thomas

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Background: The multidrug resistance (MDR) phenotype encounters a major challenge to the success of established chemotherapy in cancer patients. We hypothesized that cytotoxic medicinal plants with novel phytochemicals can overcome MDR and kill MDR-cells with similar efficacy as drug sensitive cells. Purpose: We evaluated plant extracts from an unexplored ecosystem in Egypt with unusual climate and nutrient conditions for their activity against sensitive and multidrug-resistant cancer cell lines. Material and methods/study design: Methylene chloride: methanol (1:1) and methanol: H2O (7:3) extracts of 40 plants were prepared resulting in a sum of 76 fraction containing compounds with varying polarity. The resazurin reduction assay was employed to evaluate the cytotoxicity of these extracts on five matched pairs of drug-sensitive and their drug-resistant cell lines. Flow cytometry and Western blotting was used …

AB - Background: The multidrug resistance (MDR) phenotype encounters a major challenge to the success of established chemotherapy in cancer patients. We hypothesized that cytotoxic medicinal plants with novel phytochemicals can overcome MDR and kill MDR-cells with similar efficacy as drug sensitive cells. Purpose: We evaluated plant extracts from an unexplored ecosystem in Egypt with unusual climate and nutrient conditions for their activity against sensitive and multidrug-resistant cancer cell lines. Material and methods/study design: Methylene chloride: methanol (1:1) and methanol: H2O (7:3) extracts of 40 plants were prepared resulting in a sum of 76 fraction containing compounds with varying polarity. The resazurin reduction assay was employed to evaluate the cytotoxicity of these extracts on five matched pairs of drug-sensitive and their drug-resistant cell lines. Flow cytometry and Western blotting was used …

M3 - Article

JO - Default journal

JF - Default journal

ER -

Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells

Abstract

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