Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells

Mohamed Elamir Hegazy, Sara Abdelfatah, Ahmed R. Hamed, Tarik A. Mohamed, Abdelsamed A. Elshamy, Ibrahim A. Saleh, Eman H. Reda, Nahla S. Abdel-Azim, Khaled A. Shams, Mahmoud Sakr, Yoshikazu Sugimoto, Paul Pare, Thomas Efferth

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The multidrug resistance (MDR) phenotype encounters a major challenge to the success of established chemotherapy in cancer patients. We hypothesized that cytotoxic medicinal plants with novel phytochemicals can overcome MDR and kill MDR-cells with similar efficacy as drug sensitive cells. <br><br>Purpose: We evaluated plant extracts from an unexplored ecosystem in Egypt with unusual climate and nutrient conditions for their activity against sensitive and multidrug-resistant cancer cell lines. <br><br>Material and methods/study design: Methylene chloride: methanol (1:1) and methanol: H<sub>2</sub>O (7:3) extracts of 40 plants were prepared resulting in a sum of 76 fraction containing compounds with varying polarity. The resazurin reduction assay was employed to evaluate the cytotoxicity of these extracts on five matched pairs of drug-sensitive and their drug-resistant cell lines. Flow cytometry and Western blotting was used …
Original languageEnglish
JournalDefault journal
StatePublished - Jun 1 2019

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