Cytotoxicity of 40 Egyptian plant extracts targeting mechanisms of drug-resistant cancer cells

Mohamed-Elamir F Hegazy, Sara Abdelfatah, Ahmed Hamed, Tarik A Mohamed, Abdelsameda A Elshemy, Ibrahim A Saleh, Eman H Reda, Nahla S Abdel-Azim, Khaled A Shams, Mahmoud Sakr, Yoshikazu Sugimoto, Paul Pare, Thomas Efferth

Research output: Contribution to journalArticlepeer-review

Abstract

Background The multidrug resistance (MDR) phenotype encounters a major challenge to the success of established chemotherapy in cancer patients. We hypothesized that cytotoxic medicinal plants with novel phytochemicals can overcome MDR and kill MDR-cells with similar efficacy as drug sensitive cells. Purpose We evaluated plant extracts from an unexplored ecosystem in Egypt with unusual climate and nutrient conditions for their activity against sensitive and multidrug-resistant cancer cell lines. Material and methods/ Study design Methylene chloride: methanol (1:1) and methanol: H2O (7:3) extracts of 40 plants were prepared resulting in a sum of 76 fraction containing compounds with varying polarity. The resazurin reduction assay was employed to evaluate the cytotoxicity of these extracts on five matched pairs of drug-sensitive and their drug-resistant cell lines. Flow cytometry and Western blotting was used to determine cell cycle analyses, apoptosis, and autophagy. Reactive oxygen s
Original languageEnglish
JournalPhytomedicine
StatePublished - Nov 2018

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