TY - JOUR
T1 - Cytogenetic Investigations in Bornean Rhinolophoidea Revealed Cryptic Diversity in Rhinolophus sedulus Entailing Classification of Peninsular Malaysia Specimens as a New Species
AU - Volleth, Marianne
AU - Khan, Faisal A.A.
AU - Müller, Stefan
AU - Baker, Robert J.
AU - Arenas-Viveros, Daniela
AU - Stevens, Richard D.
AU - Trifonov, Vladimir
AU - Liehr, Thomas
AU - Heller, Klaus Gerhard
AU - Sotero-Caio, Cibele G.
N1 - Funding Information:
Permission to conduct research in Malaysia was given by Economic Planning Unit UPE: 40/200/19/1466, The Sabah Parks Trustees (SP), Sarawak Forestry Department (SFD), Sara - wak Forestry Corporation (SFC) and Department of Wildlife and National Parks (DWNP). We thank all of our collaborators especially Dr. Sivanathan, E. (DWNP) and Dr. Marklarin L. (SP). We thank Prof Dato’ Dr Mohd Tajuddin Abdullah who was previously from Universiti Malaysia Sarawak (UNIMAS) in providing all the assistance from the beginning of the expedition. Trip to Malaysia was supported by Mr. James Sowell, Department Biological Sciences and Natural Science Research Laboratory, Texas Tech University, Ministry of Science, Technology and Environment of Malaysia and UNIMAS. We also would like to thank all the members of 2006 and 2010 field expeditions especially Vicki J. Swier and Molly M. Mac - donough in assisting in preparing the karyotypes. We are especially thankful for the assistance of Heath Garner (NSRL / TTU Museum). FAAK was supported by Ministry of Higher Educa - tion Malaysia, grant F07/FRGS/1878/2019. CGSC was supported by a Postdoctoral fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, during the development of this study.
Funding Information:
FAAK was supported by Ministry of Higher Educa-tion Malaysia, grant F07/FRGS/1878/2019. CGSC was supported by a Postdoctoral fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, during the development of this study.
Publisher Copyright:
© 2021 Museum and Institute of Zoology PAS. All rights reserved.
PY - 2021/6/1
Y1 - 2021/6/1
N2 - Classical and molecular cytogenetic methods were applied to study the karyotypes of one species of Hipposideridae and three taxa of the Rhinolophidae subgenus Aquias from Malaysian Borneo. Except for four chromosomal pairs with autapomorphic arm combinations, the karyotype of Coelops robinsoni was found to be similar to the closely related Aselliscus stoliczkanus. From the three Rhinolophus taxa studied, only R. trifoliatus was found to share the karyotype with conspecifics from Peninsular Malaysia. In contrast, the karyotype of R. luctus foetidus from Sarawak, Borneo differed in the composition of the Y-autosomal translocation products from the closely related R. morio from Peninsular Malaysia, formerly also a subspecies of R. luctus. Therefore, elevation to specific rank is suggested for R. l. foetidus. Examination of the chromosomal set of male R. sedulus specimens from Borneo with 2n = 45 and a Neo-X1X2Y sex chromosome system revealed extreme differences to the karyotype of specimens from Peninsular Malaysia with 2n = 28, to date also classified as R. sedulus. Therefore, with Sarawak, Borneo, as the type locality for R. sedulus, the taxon from Peninsular Malaysia is here described as a new species.
AB - Classical and molecular cytogenetic methods were applied to study the karyotypes of one species of Hipposideridae and three taxa of the Rhinolophidae subgenus Aquias from Malaysian Borneo. Except for four chromosomal pairs with autapomorphic arm combinations, the karyotype of Coelops robinsoni was found to be similar to the closely related Aselliscus stoliczkanus. From the three Rhinolophus taxa studied, only R. trifoliatus was found to share the karyotype with conspecifics from Peninsular Malaysia. In contrast, the karyotype of R. luctus foetidus from Sarawak, Borneo differed in the composition of the Y-autosomal translocation products from the closely related R. morio from Peninsular Malaysia, formerly also a subspecies of R. luctus. Therefore, elevation to specific rank is suggested for R. l. foetidus. Examination of the chromosomal set of male R. sedulus specimens from Borneo with 2n = 45 and a Neo-X1X2Y sex chromosome system revealed extreme differences to the karyotype of specimens from Peninsular Malaysia with 2n = 28, to date also classified as R. sedulus. Therefore, with Sarawak, Borneo, as the type locality for R. sedulus, the taxon from Peninsular Malaysia is here described as a new species.
KW - cytotaxonomy
KW - fluorescence in-situ hybridization
KW - sex-autosome translocations
UR - http://www.scopus.com/inward/record.url?scp=85109965151&partnerID=8YFLogxK
U2 - 10.3161/15081109ACC2021.23.1.001
DO - 10.3161/15081109ACC2021.23.1.001
M3 - Article
AN - SCOPUS:85109965151
SN - 1508-1109
VL - 23
SP - 1
EP - 20
JO - Acta Chiropterologica
JF - Acta Chiropterologica
IS - 1
ER -