CYP1A Protein Expression and Catalytic Activity in Double-crested Cormorants Experimentally Exposed to Deepwater Horizon Mississippi Canyon 252 Oil

Courtney Alexander; Michael Hooper; Dave Cacela; Kim Smelker; Caleshia Calvin; Karen Dean; Steve Bursian; Fred Cunningham; Katie Hanson-Dorr; Katherine Horak; John Isanhart; Jane Link; Susan Shriner; Celine Godard

CYP1A Protein Expression and Catalytic Activity in Double-crested Cormorants Experimentally Exposed to Deepwater Horizon Mississippi Canyon 252 Oil

Courtney Alexander, Michael Hooper, Dave Cacela, Kim Smelker, Caleshia Calvin, Karen Dean, Steve Bursian, Fred Cunningham, Katie Hanson-Dorr, Katherine Horak, John Isanhart, Jane Link, Susan Shriner, Celine Godard

Environmental Toxicology

Research output: Contribution to journal › Article › peer-review

Abstract

Double-crested cormorants (Phalacrocorax auritus, DCCO) were orally exposed to Deepwater Horizon Mississippi Canyon 252 (DWH) oil to investigate oil-induced toxicological impacts. Livers were collected for multiple analyses including cytochrome P4501A (CYP1A) enzymatic activity and protein expression. CYP1A catalyzes the biotransformation and subsequent activation of polycyclic aromatic hydrocarbons (PAHs) found in oil. CYP1A induction is a widely used biomarker of exposure to specific contaminants including PAHs in avian, as well as laboratory and other wildlife species. CYP1A enzymatic activity was measured by alkoxyresorufin O-dealkylase (AROD) assays. Activities specific to the O-dealkylation of four resorufin ethers are reported: benzyloxyresorufin O-debenzylase (BROD), ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and pentoxyresorufin O-depentylase (PROD). CYP1A protein expression was measured by western blot analysis with a CYP1A1 mouse monoclonal

Original language	English
Pages (from-to)	79-86
Journal	Ecotoxicology and Environmental Safety
State	Published - Jan 2017

Cite this

Alexander, C., Hooper, M., Cacela, D., Smelker, K., Calvin, C., Dean, K., Bursian, S., Cunningham, F., Hanson-Dorr, K., Horak, K., Isanhart, J., Link, J., Shriner, S., & Godard, C. (2017). CYP1A Protein Expression and Catalytic Activity in Double-crested Cormorants Experimentally Exposed to Deepwater Horizon Mississippi Canyon 252 Oil. Ecotoxicology and Environmental Safety, 79-86.

@article{0e2d1b1980184ca4b29e82a1c71b1e67,

title = "CYP1A Protein Expression and Catalytic Activity in Double-crested Cormorants Experimentally Exposed to Deepwater Horizon Mississippi Canyon 252 Oil",

abstract = "Double-crested cormorants (Phalacrocorax auritus, DCCO) were orally exposed to Deepwater Horizon Mississippi Canyon 252 (DWH) oil to investigate oil-induced toxicological impacts. Livers were collected for multiple analyses including cytochrome P4501A (CYP1A) enzymatic activity and protein expression. CYP1A catalyzes the biotransformation and subsequent activation of polycyclic aromatic hydrocarbons (PAHs) found in oil. CYP1A induction is a widely used biomarker of exposure to specific contaminants including PAHs in avian, as well as laboratory and other wildlife species. CYP1A enzymatic activity was measured by alkoxyresorufin O-dealkylase (AROD) assays. Activities specific to the O-dealkylation of four resorufin ethers are reported: benzyloxyresorufin O-debenzylase (BROD), ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and pentoxyresorufin O-depentylase (PROD). CYP1A protein expression was measured by western blot analysis with a CYP1A1 mouse monoclonal",

author = "Courtney Alexander and Michael Hooper and Dave Cacela and Kim Smelker and Caleshia Calvin and Karen Dean and Steve Bursian and Fred Cunningham and Katie Hanson-Dorr and Katherine Horak and John Isanhart and Jane Link and Susan Shriner and Celine Godard",

year = "2017",

month = jan,

language = "English",

pages = "79--86",

journal = "Ecotoxicology and Environmental Safety",

}

Alexander, C, Hooper, M, Cacela, D, Smelker, K, Calvin, C, Dean, K, Bursian, S, Cunningham, F, Hanson-Dorr, K, Horak, K, Isanhart, J, Link, J, Shriner, S & Godard, C 2017, 'CYP1A Protein Expression and Catalytic Activity in Double-crested Cormorants Experimentally Exposed to Deepwater Horizon Mississippi Canyon 252 Oil', Ecotoxicology and Environmental Safety, pp. 79-86.

TY - JOUR

T1 - CYP1A Protein Expression and Catalytic Activity in Double-crested Cormorants Experimentally Exposed to Deepwater Horizon Mississippi Canyon 252 Oil

AU - Alexander, Courtney

AU - Hooper, Michael

AU - Cacela, Dave

AU - Smelker, Kim

AU - Calvin, Caleshia

AU - Dean, Karen

AU - Bursian, Steve

AU - Cunningham, Fred

AU - Hanson-Dorr, Katie

AU - Horak, Katherine

AU - Isanhart, John

AU - Link, Jane

AU - Shriner, Susan

AU - Godard, Celine

PY - 2017/1

Y1 - 2017/1

N2 - Double-crested cormorants (Phalacrocorax auritus, DCCO) were orally exposed to Deepwater Horizon Mississippi Canyon 252 (DWH) oil to investigate oil-induced toxicological impacts. Livers were collected for multiple analyses including cytochrome P4501A (CYP1A) enzymatic activity and protein expression. CYP1A catalyzes the biotransformation and subsequent activation of polycyclic aromatic hydrocarbons (PAHs) found in oil. CYP1A induction is a widely used biomarker of exposure to specific contaminants including PAHs in avian, as well as laboratory and other wildlife species. CYP1A enzymatic activity was measured by alkoxyresorufin O-dealkylase (AROD) assays. Activities specific to the O-dealkylation of four resorufin ethers are reported: benzyloxyresorufin O-debenzylase (BROD), ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and pentoxyresorufin O-depentylase (PROD). CYP1A protein expression was measured by western blot analysis with a CYP1A1 mouse monoclonal

AB - Double-crested cormorants (Phalacrocorax auritus, DCCO) were orally exposed to Deepwater Horizon Mississippi Canyon 252 (DWH) oil to investigate oil-induced toxicological impacts. Livers were collected for multiple analyses including cytochrome P4501A (CYP1A) enzymatic activity and protein expression. CYP1A catalyzes the biotransformation and subsequent activation of polycyclic aromatic hydrocarbons (PAHs) found in oil. CYP1A induction is a widely used biomarker of exposure to specific contaminants including PAHs in avian, as well as laboratory and other wildlife species. CYP1A enzymatic activity was measured by alkoxyresorufin O-dealkylase (AROD) assays. Activities specific to the O-dealkylation of four resorufin ethers are reported: benzyloxyresorufin O-debenzylase (BROD), ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and pentoxyresorufin O-depentylase (PROD). CYP1A protein expression was measured by western blot analysis with a CYP1A1 mouse monoclonal

M3 - Article

SP - 79

EP - 86

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

ER -

CYP1A Protein Expression and Catalytic Activity in Double-crested Cormorants Experimentally Exposed to Deepwater Horizon Mississippi Canyon 252 Oil

Abstract

Fingerprint

Cite this