CYP1A Protein Expression and Catalytic Activity in Double-crested Cormorants Experimentally Exposed to Deepwater Horizon Mississippi Canyon 252 Oil

Courtney Alexander, Michael Hooper, Dave Cacela, Kim Smelker, Caleshia Calvin, Karen Dean, Steve Bursian, Fred Cunningham, Katie Hanson-Dorr, Katherine Horak, John Isanhart, Jane Link, Susan Shriner, Celine Godard

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Abstract

Double-crested cormorants (Phalacrocorax auritus, DCCO) were orally exposed to Deepwater Horizon Mississippi Canyon 252 (DWH) oil to investigate oil-induced toxicological impacts. Livers were collected for multiple analyses including cytochrome P4501A (CYP1A) enzymatic activity and protein expression. CYP1A catalyzes the biotransformation and subsequent activation of polycyclic aromatic hydrocarbons (PAHs) found in oil. CYP1A induction is a widely used biomarker of exposure to specific contaminants including PAHs in avian, as well as laboratory and other wildlife species. CYP1A enzymatic activity was measured by alkoxyresorufin O-dealkylase (AROD) assays. Activities specific to the O-dealkylation of four resorufin ethers are reported: benzyloxyresorufin O-debenzylase (BROD), ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and pentoxyresorufin O-depentylase (PROD). CYP1A protein expression was measured by western blot analysis with a CYP1A1 mouse monoclonal
Original languageEnglish
Pages (from-to)79-86
JournalEcotoxicology and Environmental Safety
StatePublished - Jan 2017

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    Alexander, C., Hooper, M., Cacela, D., Smelker, K., Calvin, C., Dean, K., Bursian, S., Cunningham, F., Hanson-Dorr, K., Horak, K., Isanhart, J., Link, J., Shriner, S., & Godard, C. (2017). CYP1A Protein Expression and Catalytic Activity in Double-crested Cormorants Experimentally Exposed to Deepwater Horizon Mississippi Canyon 252 Oil. Ecotoxicology and Environmental Safety, 79-86.