Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine

H. S. Gill, J. Söderholm, M. R. Prausnitz, M. Sällberg

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

The skin is potentially an excellent organ for vaccine delivery because of accessibility and the presence of immune cells. However, no simple and inexpensive cutaneous vaccination method is available. Micron-scale needles coated with DNA were tested as a simple, inexpensive device for skin delivery. Vaccination with a plasmid encoding hepatitis C virus nonstructural 3/4A protein using microneedles effectively primed specific cytotoxic T lymphocytes (CTLs). Importantly, the minimally invasive microneedles were as efficient in priming CTLs as more complicated or invasive delivery techniques, such as gene gun and hypodermic needles. Thus, microneedles may offer a promising technology for DNA vaccination.

Original languageEnglish
Pages (from-to)811-814
Number of pages4
JournalGene Therapy
Volume17
Issue number6
DOIs
StatePublished - Jun 2010

Keywords

  • Cellular immune response
  • DNA vaccine
  • DNA-coated microneedle
  • Hepatitis C virus
  • Microfabricated needle

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