Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine

H. S. Gill; J. Söderholm; M. R. Prausnitz; M. Sällberg

doi:10.1038/gt.2010.22

Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine

H. S. Gill, J. Söderholm, M. R. Prausnitz, M. Sällberg

Chemical Engineering

Research output: Contribution to journal › Article › peer-review

137 Scopus citations

Abstract

The skin is potentially an excellent organ for vaccine delivery because of accessibility and the presence of immune cells. However, no simple and inexpensive cutaneous vaccination method is available. Micron-scale needles coated with DNA were tested as a simple, inexpensive device for skin delivery. Vaccination with a plasmid encoding hepatitis C virus nonstructural 3/4A protein using microneedles effectively primed specific cytotoxic T lymphocytes (CTLs). Importantly, the minimally invasive microneedles were as efficient in priming CTLs as more complicated or invasive delivery techniques, such as gene gun and hypodermic needles. Thus, microneedles may offer a promising technology for DNA vaccination.

Original language	English
Pages (from-to)	811-814
Number of pages	4
Journal	Gene Therapy
Volume	17
Issue number	6
DOIs	https://doi.org/10.1038/gt.2010.22
State	Published - Jun 2010

Keywords

Cellular immune response
DNA vaccine
DNA-coated microneedle
Hepatitis C virus
Microfabricated needle

Access to Document

10.1038/gt.2010.22

Cite this

@article{dcdf9be1b0bb4042ab7c0e2fa2f32546,

title = "Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine",

abstract = "The skin is potentially an excellent organ for vaccine delivery because of accessibility and the presence of immune cells. However, no simple and inexpensive cutaneous vaccination method is available. Micron-scale needles coated with DNA were tested as a simple, inexpensive device for skin delivery. Vaccination with a plasmid encoding hepatitis C virus nonstructural 3/4A protein using microneedles effectively primed specific cytotoxic T lymphocytes (CTLs). Importantly, the minimally invasive microneedles were as efficient in priming CTLs as more complicated or invasive delivery techniques, such as gene gun and hypodermic needles. Thus, microneedles may offer a promising technology for DNA vaccination.",

keywords = "Cellular immune response, DNA vaccine, DNA-coated microneedle, Hepatitis C virus, Microfabricated needle",

author = "Gill, {H. S.} and J. S{\"o}derholm and Prausnitz, {M. R.} and M. S{\"a}llberg",

note = "Funding Information: This study was supported by the Swedish Science Council, the Cancer Foundation, Stockholm County Council, US National Institutes of Health, and Robert M Nerem Travel Award to HSG.",

year = "2010",

month = jun,

doi = "10.1038/gt.2010.22",

language = "English",

volume = "17",

pages = "811--814",

journal = "Gene Therapy",

issn = "0969-7128",

number = "6",

}

TY - JOUR

T1 - Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine

AU - Gill, H. S.

AU - Söderholm, J.

AU - Prausnitz, M. R.

AU - Sällberg, M.

N1 - Funding Information: This study was supported by the Swedish Science Council, the Cancer Foundation, Stockholm County Council, US National Institutes of Health, and Robert M Nerem Travel Award to HSG.

PY - 2010/6

Y1 - 2010/6

N2 - The skin is potentially an excellent organ for vaccine delivery because of accessibility and the presence of immune cells. However, no simple and inexpensive cutaneous vaccination method is available. Micron-scale needles coated with DNA were tested as a simple, inexpensive device for skin delivery. Vaccination with a plasmid encoding hepatitis C virus nonstructural 3/4A protein using microneedles effectively primed specific cytotoxic T lymphocytes (CTLs). Importantly, the minimally invasive microneedles were as efficient in priming CTLs as more complicated or invasive delivery techniques, such as gene gun and hypodermic needles. Thus, microneedles may offer a promising technology for DNA vaccination.

AB - The skin is potentially an excellent organ for vaccine delivery because of accessibility and the presence of immune cells. However, no simple and inexpensive cutaneous vaccination method is available. Micron-scale needles coated with DNA were tested as a simple, inexpensive device for skin delivery. Vaccination with a plasmid encoding hepatitis C virus nonstructural 3/4A protein using microneedles effectively primed specific cytotoxic T lymphocytes (CTLs). Importantly, the minimally invasive microneedles were as efficient in priming CTLs as more complicated or invasive delivery techniques, such as gene gun and hypodermic needles. Thus, microneedles may offer a promising technology for DNA vaccination.

KW - Cellular immune response

KW - DNA vaccine

KW - DNA-coated microneedle

KW - Hepatitis C virus

KW - Microfabricated needle

UR - http://www.scopus.com/inward/record.url?scp=77953479878&partnerID=8YFLogxK

U2 - 10.1038/gt.2010.22

DO - 10.1038/gt.2010.22

M3 - Article

C2 - 20200562

AN - SCOPUS:77953479878

SN - 0969-7128

VL - 17

SP - 811

EP - 814

JO - Gene Therapy

JF - Gene Therapy

IS - 6

ER -

Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this