The objective of this study was to identify mutants of upland cottonseed (Gossypium hirsutum L.) with reduced levels of palmitic acid (C16:0) in the seed. The five parental populations that had been exposed to Ethyl MethaneSulfonate in 1997 or 2002 were initially screened for either herbicide tolerance or divergent fiber quality. Fatty acid composition were determined for five seed bulks from 140 M5 mutant lines in 2009 and 100 M6 partial seeds in 2010. Mutants with reduced levels of palmitic acid levels were isolated from TAM 94L-25 (PI 631440), Acala 1517-99 (Reg. No. CV-115, PI 612326) and TTU SCM3-7-3 (Reg. No.GS-4, PI 657942). Thirteen M6 single seeds with reduced levels of palmitic acid (17.9–19.6%) were intercrossed in the greenhouse. Ten of the single seed selections were designated as potential male parents and randomly crossed to three potential female parents to generate F1 plants and 21 F2 populations for allele testing. Based on these tests, five F2 populations with reduced levels of low palmitic acid, were selected for further evaluation. The development of mutant lines with reduced levels of palmitic acid in upland cotton was submitted for proprietary protection under U.S. Patent No. 20160222399.
- Chemical mutagenesis
- Fatty acid composition of cottonseed oil
- Reduced levels of palmitic acid