Abstract
An optical fiber biosensor to detect calpastatin has been investigated as a preliminary step in developing tenderness detection instrumentation. Longissimus dorsi samples were taken from beef carcasses (n = 21) at 0, 24, 36 and 48 h postmortem. Muscle homogenates were assayed for calpastatin activity using traditional methods and an optical fiber biosensor. Warner-Bratzler shear force was also performed on a steak from each carcass at 14 d postmortem. Results demonstrated that the measurements with highest correlation between traditional calpastatin assays and optical biosensor readings were taken at 48 h postmortem (r = 0.597, P ≤ 0.01), suggesting that this is the best time for use of this biosensor in an on-line grading system. This research further advances the development of a calpastatin biosensor and would be useful in laboratory determination of the presence of biologically active calpastatin concentrations.
Original language | English |
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Pages (from-to) | 1429-1434 |
Number of pages | 6 |
Journal | Biosensors and Bioelectronics |
Volume | 23 |
Issue number | 10 |
DOIs | |
State | Published - May 15 2008 |
Keywords
- Beef
- Biosensor
- Calpastatin
- Fluorescence
- Tenderness