Construction of a sugar beet BAC library from a hybrid with diverse traits

J. M. McGrath, R. Scott Shaw, Benildo G. De Los Reyes, John J. Weiland

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

A bacterial artificial chromosome (BAC) library of the 750-Mbp sugar beet genome represented in hybrid US H20 was constructed from Hind III-digested DNA, with an average insert size of 120 kbp. US H20 is a variety grown in the eastern United States. It exhibits heterosis for emergence and yield, presumably because of its hybridity between eastern and western US germplasm sources. Filter arrays were used to assess the abundance and distribution of particular nucleotide sequences. An rRNA gene probe found that 1.2% of the library carried sequences similar to these highly repetitive and conserved sequences. A simple sequence repeat element (CA)8 thought to be predominantly distributed throughout centromere regions of all chromosomes was present in 1.7% of clones. For more than half of the 28 randomly chosen expressed sequence tags (ESTs) used as probes, a higher-than-expected number of single-copy hybridization signals was observed. Assuming 6x genome coverage, this suggests that many duplicate genes exist in the beet genome.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
JournalPlant Molecular Biology Reporter
Volume22
Issue number1
DOIs
StatePublished - Mar 2004

Keywords

  • BAC library
  • Gene copy number
  • Genetic resource
  • Physical mapping
  • Sugar beet

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