Computer-assisted (or automated) sperm analysis (CASA) is an objective way to evaluate spermatozoal motility. CASA employs the use of a microscope, a video camera, and computer software for the analysis. The system records multiple images of sperm movement to provide data that the computer system then uses to "connect the dots" resulting in a linear path of individual sperm movement. A special microscope slide is recommended for use in the CASA system that has a predefined volume. The chapter discusses the step by step procedures to perform the CASA, and the equipment and supplied needed. Motility estimation is performed slightly differently with different CASA machines based on the age of the software/hardware and the manufacturer. The chapter also discusses some methods to estimate spermatozoal motility.
- Computer-assisted sperm analysis (CASA)
- Microscope slide
- Spermatozoal motility