Comprehensive study of unexpected microscope condensers formed in sample arrangements commonly used in optical microscopy

Darshan B. Desai, Mabkhoot Mudith S. Aldawsari, Bandar Mohammed H. Alharbi, Sanchari Sen, Luis Grave De Peralta

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We show that various setups for optical microscopy which are commonly used in biomedical laboratories behave like efficient microscope condensers that are responsible for observed subwavelength resolution. We present a series of experiments and simulations that reveal how inclined illumination from such unexpected condensers occurs when the sample is perpendicularly illuminated by a microscope's built-in white-light source. In addition, we demonstrate an inexpensive add-on optical module that serves as an efficient and lightweight microscope condenser. Using such add-on optical module in combination with a low-numerical-aperture objective lens and Fourier plane imaging microscopy technique, we demonstrate detection of photonic crystals with a period nearly eight times smaller than the Rayleigh resolution limit.

Original languageEnglish
Pages (from-to)7781-7788
Number of pages8
JournalApplied Optics
Volume54
Issue number25
DOIs
StatePublished - Sep 1 2015

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