TY - JOUR
T1 - Complexation trends and binding constants between dextrin oligomers and small molecules as measured through affinity capillary electrophoresis
AU - Hoffmann, Julia
AU - Mechref, Yehia
AU - Cassely, Aaron
AU - Novotny, Milos V.
PY - 2003/9
Y1 - 2003/9
N2 - Quantitative aspects of a previously reported pre-equilibration affinity/capillary electrophoretic methodology (Hong, M., et al., Anal. Chem. 1998, 70, 3590-3597) were studied using fluorescently labeled oligosaccharides and analgesic drugs. Frontal analysis capillary electrophoresis (FA-CE) was investigated for these interaction systems. In order to fulfill certain FA requirements, the analysis conditions of the sugar-drug combinations had to be adjusted individually. Only after the establishment of these conditions, acceptable binding data (association constants) could be obtained. The constants are in the range of weak hydrogen bonding and are comparable to the constants that were independently obtained through 1H nuclear magnetic resonance (NMR) spectroscopy.
AB - Quantitative aspects of a previously reported pre-equilibration affinity/capillary electrophoretic methodology (Hong, M., et al., Anal. Chem. 1998, 70, 3590-3597) were studied using fluorescently labeled oligosaccharides and analgesic drugs. Frontal analysis capillary electrophoresis (FA-CE) was investigated for these interaction systems. In order to fulfill certain FA requirements, the analysis conditions of the sugar-drug combinations had to be adjusted individually. Only after the establishment of these conditions, acceptable binding data (association constants) could be obtained. The constants are in the range of weak hydrogen bonding and are comparable to the constants that were independently obtained through 1H nuclear magnetic resonance (NMR) spectroscopy.
KW - Affinity capillary electrophoresis
KW - Dextrin
KW - Model pharmaceutical
KW - Spectroscopic detection
UR - http://www.scopus.com/inward/record.url?scp=0345873409&partnerID=8YFLogxK
U2 - 10.1002/elps.200305562
DO - 10.1002/elps.200305562
M3 - Article
C2 - 12973794
AN - SCOPUS:0345873409
SN - 0173-0835
VL - 24
SP - 2914
EP - 2923
JO - ELECTROPHORESIS
JF - ELECTROPHORESIS
IS - 17
ER -