Complex roles of hybrid lipids in the composition, order, and size of lipid membrane domains

Ebrahim Hassan-Zadeh; Eda Baykal-Caglar; Mohammad Alwarawrah; Juyang Huang

Complex roles of hybrid lipids in the composition, order, and size of lipid membrane domains

Ebrahim Hassan-Zadeh, Eda Baykal-Caglar, Mohammad Alwarawrah, Juyang Huang

Physics

Research output: Contribution to journal › Article › peer-review

Abstract

Hybrid lipids (HL) are phospholipids with one saturated chain and one unsaturated chain. HL are hypothesized to act as linactants (i.e., 2D surfactants) in cell membranes, reducing line tension and creating nanoscopic lipid domains. Here we compare three hybrid lipids of different chain unsaturation (16:0-18:1PC (POPC), 16:0-18:2PC (PLPC), and 16:0-20:4PC (PAPC)) in their abilities to alter the composition, line tension, order, and compactness of lipid domains. We found that the liquid-ordered (L o ) and liquid-disordered (L d ) lipid domains in PAPC/di18:0PC(DSPC)/ cholesterol and PLPC/DSPC/cholesterol mixtures are micrometer-sized, and only the POPC/DSPC/cholesterol system has nanoscopic domains. The results indicate that some HLs with polyunsaturated chains are not linactants, and the monounsaturated POPC displays both properties of weak linactants and "L d -phase" lipids such as di18:1PC (DOPC). The obtained phase boundaries from giant unilamellar vesicles (GUV) show that both POP

Original language	English
Pages (from-to)	1361-1369
Journal	Langmuir
State	Published - Feb 11 2014

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title = "Complex roles of hybrid lipids in the composition, order, and size of lipid membrane domains",

abstract = "Hybrid lipids (HL) are phospholipids with one saturated chain and one unsaturated chain. HL are hypothesized to act as linactants (i.e., 2D surfactants) in cell membranes, reducing line tension and creating nanoscopic lipid domains. Here we compare three hybrid lipids of different chain unsaturation (16:0-18:1PC (POPC), 16:0-18:2PC (PLPC), and 16:0-20:4PC (PAPC)) in their abilities to alter the composition, line tension, order, and compactness of lipid domains. We found that the liquid-ordered (L o ) and liquid-disordered (L d ) lipid domains in PAPC/di18:0PC(DSPC)/ cholesterol and PLPC/DSPC/cholesterol mixtures are micrometer-sized, and only the POPC/DSPC/cholesterol system has nanoscopic domains. The results indicate that some HLs with polyunsaturated chains are not linactants, and the monounsaturated POPC displays both properties of weak linactants and {"}L d -phase{"} lipids such as di18:1PC (DOPC). The obtained phase boundaries from giant unilamellar vesicles (GUV) show that both POP",

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year = "2014",

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journal = "Langmuir",

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T1 - Complex roles of hybrid lipids in the composition, order, and size of lipid membrane domains

AU - Hassan-Zadeh, Ebrahim

AU - Baykal-Caglar, Eda

AU - Alwarawrah, Mohammad

AU - Huang, Juyang

PY - 2014/2/11

Y1 - 2014/2/11

N2 - Hybrid lipids (HL) are phospholipids with one saturated chain and one unsaturated chain. HL are hypothesized to act as linactants (i.e., 2D surfactants) in cell membranes, reducing line tension and creating nanoscopic lipid domains. Here we compare three hybrid lipids of different chain unsaturation (16:0-18:1PC (POPC), 16:0-18:2PC (PLPC), and 16:0-20:4PC (PAPC)) in their abilities to alter the composition, line tension, order, and compactness of lipid domains. We found that the liquid-ordered (L o ) and liquid-disordered (L d ) lipid domains in PAPC/di18:0PC(DSPC)/ cholesterol and PLPC/DSPC/cholesterol mixtures are micrometer-sized, and only the POPC/DSPC/cholesterol system has nanoscopic domains. The results indicate that some HLs with polyunsaturated chains are not linactants, and the monounsaturated POPC displays both properties of weak linactants and "L d -phase" lipids such as di18:1PC (DOPC). The obtained phase boundaries from giant unilamellar vesicles (GUV) show that both POP

AB - Hybrid lipids (HL) are phospholipids with one saturated chain and one unsaturated chain. HL are hypothesized to act as linactants (i.e., 2D surfactants) in cell membranes, reducing line tension and creating nanoscopic lipid domains. Here we compare three hybrid lipids of different chain unsaturation (16:0-18:1PC (POPC), 16:0-18:2PC (PLPC), and 16:0-20:4PC (PAPC)) in their abilities to alter the composition, line tension, order, and compactness of lipid domains. We found that the liquid-ordered (L o ) and liquid-disordered (L d ) lipid domains in PAPC/di18:0PC(DSPC)/ cholesterol and PLPC/DSPC/cholesterol mixtures are micrometer-sized, and only the POPC/DSPC/cholesterol system has nanoscopic domains. The results indicate that some HLs with polyunsaturated chains are not linactants, and the monounsaturated POPC displays both properties of weak linactants and "L d -phase" lipids such as di18:1PC (DOPC). The obtained phase boundaries from giant unilamellar vesicles (GUV) show that both POP

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JO - Langmuir

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