Complex roles of hybrid lipids in the composition, order, and size of lipid membrane domains

Ebrahim Hassan-Zadeh, Eda Baykal-Caglar, Mohammad Alwarawrah, Juyang Huang

Research output: Contribution to journalArticlepeer-review

Abstract

Hybrid lipids (HL) are phospholipids with one saturated chain and one unsaturated chain. HL are hypothesized to act as linactants (i.e., 2D surfactants) in cell membranes, reducing line tension and creating nanoscopic lipid domains. Here we compare three hybrid lipids of different chain unsaturation (16:0-18:1PC (POPC), 16:0-18:2PC (PLPC), and 16:0-20:4PC (PAPC)) in their abilities to alter the composition, line tension, order, and compactness of lipid domains. We found that the liquid-ordered (L o ) and liquid-disordered (L d ) lipid domains in PAPC/di18:0PC(DSPC)/ cholesterol and PLPC/DSPC/cholesterol mixtures are micrometer-sized, and only the POPC/DSPC/cholesterol system has nanoscopic domains. The results indicate that some HLs with polyunsaturated chains are not linactants, and the monounsaturated POPC displays both properties of weak linactants and "L d -phase" lipids such as di18:1PC (DOPC). The obtained phase boundaries from giant unilamellar vesicles (GUV) show that both POP
Original languageEnglish
Pages (from-to)1361-1369
JournalLangmuir
StatePublished - Feb 11 2014

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