Comparison of Inlet Geometry in Microfluidic Cell Affinity Chromatography

Peng Li, Yu Tian, Dimitrios Pappas

Research output: Contribution to journalArticlepeer-review


Cell separation based on microfluidic affinity chromatography is a widely used methodology in cell analysis research when rapid separations with high purity are needed. Several successful examples have been reported with high separation efficiency and purity; however, cell capture at the inlet area and inlet design have not been extensively described or studied. The most common inlets;used to connect the microfluidic chip to pumps, tubing, etc.;are vertical (top-loading) inlets and parallel (in-line) inlets. In this work, we investigated the cell capture behavior near the affinity chip inlet area and compared the different performances of vertical inlet devices and parallel inlet devices. Vertical inlet devices showed significant cell capture capability near the inlet area, which led to the formation of cell blockages as the separation progressed. Cell density near the inlet area was much higher than that in the remaining channel, whereas for parallel inlet chips cell density at the i
Original languageEnglish
Pages (from-to)774-781
JournalAnalytical Chemistry
StatePublished - 2011


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