TY - JOUR
T1 - Cloning of a galacturonic acid uptake gene from Erwinia chrysanthemi EC16
AU - Freeman, Thomas L.
AU - San Francisco, Michael J.D.
N1 - Funding Information:
Portions of this research formed part of the M.S. thesis of T.L.F. (Texas Tech University, 1993) and were supported by grants DCB-9020905 and 92-37303-7617 from the National Science Foundation and U.S. Department of Agriculture, respectively. The work of Brian Haseloff in the complementation assays and Michael Melkus in reading the manuscript is acknowledged. We thank Fredrique van Gijsegem for strain ERH215 and plasmid pULBll4 and Noel Keen for the E. chrysanthemi EC16 genomic library.
PY - 1994/5/1
Y1 - 1994/5/1
N2 - A 3.4 kb fragment of Erwinia chrysanthemi EC16 DNA capable of complementing galacturonic acid uptake mutants (exuT) was identified and cloned into a multicopy vector. In E. chrysanthemi B374 exuT mutants, the cloned DNA provided for growth of the mutant strains on galacturonic acid by complementing the galacturonic acid uptake defect. Alkaline phosphatase (phoA) gene fusions with the cloned DNA suggested that most of the cloned DNA was necessary for complementation of exuT mutant strains. Using anti-alkaline phosphatase antibody, a hybrid ExuT-PhoA protein was localized to the membrane fraction of the bacterium.
AB - A 3.4 kb fragment of Erwinia chrysanthemi EC16 DNA capable of complementing galacturonic acid uptake mutants (exuT) was identified and cloned into a multicopy vector. In E. chrysanthemi B374 exuT mutants, the cloned DNA provided for growth of the mutant strains on galacturonic acid by complementing the galacturonic acid uptake defect. Alkaline phosphatase (phoA) gene fusions with the cloned DNA suggested that most of the cloned DNA was necessary for complementation of exuT mutant strains. Using anti-alkaline phosphatase antibody, a hybrid ExuT-PhoA protein was localized to the membrane fraction of the bacterium.
KW - Erwinia chrysanthemi
KW - Galacturonic acid transport
KW - Pectate lyase
KW - exuT
UR - http://www.scopus.com/inward/record.url?scp=0028200388&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1994.tb06810.x
DO - 10.1111/j.1574-6968.1994.tb06810.x
M3 - Article
AN - SCOPUS:0028200388
SN - 0378-1097
VL - 118
SP - 101
EP - 106
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1-2
ER -