Cloning and expression of an Arabidopsis gene encoding a putative peroxisomal ascorbate peroxidase

Hong Zhang; Jing Wang; Utah Nickel; Randy D. Allen; Howard M. Goodman

doi:10.1023/A:1005814109732

Cloning and expression of an Arabidopsis gene encoding a putative peroxisomal ascorbate peroxidase

Hong Zhang, Jing Wang, Utah Nickel, Randy D. Allen, Howard M. Goodman

Biological Sciences

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

An Arabidopsis 14-3-3 protein, AFT1, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. One of the candidate proteins, APX3, was identified as a putative peroxisomal membrane-bound ascorbate peroxidase. Ascorbate peroxidases are important defense enzymes that protect plant cells from oxidative stress damage. DNA blot analysis indicates that APX3 is encoded by a single-copy gene in the Arabidopsis genome. RNA blot analyses show that APX3 transcript levels increase slightly in response to cold, UV light, and treatments with hydrogen peroxide and paraquat. The activity of APX3 in Arabidopsis may be controlled in two ways: its enzymatic activity through protein-protein interactions and its transcription by transcriptional or posttranscriptional regulation.

Original language	English
Pages (from-to)	967-971
Number of pages	5
Journal	Plant molecular biology
Volume	34
Issue number	6
DOIs	https://doi.org/10.1023/A:1005814109732
State	Published - Aug 1997

Keywords

14-3-3 protein
Arabidopsis
Ascorbate peroxidase
Oxidative stress
Peroxisome

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title = "Cloning and expression of an Arabidopsis gene encoding a putative peroxisomal ascorbate peroxidase",

abstract = "An Arabidopsis 14-3-3 protein, AFT1, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. One of the candidate proteins, APX3, was identified as a putative peroxisomal membrane-bound ascorbate peroxidase. Ascorbate peroxidases are important defense enzymes that protect plant cells from oxidative stress damage. DNA blot analysis indicates that APX3 is encoded by a single-copy gene in the Arabidopsis genome. RNA blot analyses show that APX3 transcript levels increase slightly in response to cold, UV light, and treatments with hydrogen peroxide and paraquat. The activity of APX3 in Arabidopsis may be controlled in two ways: its enzymatic activity through protein-protein interactions and its transcription by transcriptional or posttranscriptional regulation.",

keywords = "14-3-3 protein, Arabidopsis, Ascorbate peroxidase, Oxidative stress, Peroxisome",

author = "Hong Zhang and Jing Wang and Utah Nickel and Allen, {Randy D.} and Goodman, {Howard M.}",

note = "Funding Information: We thank Russ Finley and Roger Brent for advice, yeast strains and plasmids used in the two-hybrid screening experiments. We thank Sheryl Schake and Robert Webb for help in analysing APX activities, and we also thank the National Science Foundation for supporting U.N. through a NSF-REU grant to Texas Tech University. This research was supported by grants from Hoechst AG (Frankfurt, Germany) to H.M.G., the research enhancement award from Texas Tech University to H.Z., and Human Frontiers in Sciences to R.D.A.",

year = "1997",

month = aug,

doi = "10.1023/A:1005814109732",

language = "English",

volume = "34",

pages = "967--971",

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T1 - Cloning and expression of an Arabidopsis gene encoding a putative peroxisomal ascorbate peroxidase

AU - Zhang, Hong

AU - Wang, Jing

AU - Nickel, Utah

AU - Allen, Randy D.

AU - Goodman, Howard M.

N1 - Funding Information: We thank Russ Finley and Roger Brent for advice, yeast strains and plasmids used in the two-hybrid screening experiments. We thank Sheryl Schake and Robert Webb for help in analysing APX activities, and we also thank the National Science Foundation for supporting U.N. through a NSF-REU grant to Texas Tech University. This research was supported by grants from Hoechst AG (Frankfurt, Germany) to H.M.G., the research enhancement award from Texas Tech University to H.Z., and Human Frontiers in Sciences to R.D.A.

PY - 1997/8

Y1 - 1997/8

N2 - An Arabidopsis 14-3-3 protein, AFT1, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. One of the candidate proteins, APX3, was identified as a putative peroxisomal membrane-bound ascorbate peroxidase. Ascorbate peroxidases are important defense enzymes that protect plant cells from oxidative stress damage. DNA blot analysis indicates that APX3 is encoded by a single-copy gene in the Arabidopsis genome. RNA blot analyses show that APX3 transcript levels increase slightly in response to cold, UV light, and treatments with hydrogen peroxide and paraquat. The activity of APX3 in Arabidopsis may be controlled in two ways: its enzymatic activity through protein-protein interactions and its transcription by transcriptional or posttranscriptional regulation.

AB - An Arabidopsis 14-3-3 protein, AFT1, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. One of the candidate proteins, APX3, was identified as a putative peroxisomal membrane-bound ascorbate peroxidase. Ascorbate peroxidases are important defense enzymes that protect plant cells from oxidative stress damage. DNA blot analysis indicates that APX3 is encoded by a single-copy gene in the Arabidopsis genome. RNA blot analyses show that APX3 transcript levels increase slightly in response to cold, UV light, and treatments with hydrogen peroxide and paraquat. The activity of APX3 in Arabidopsis may be controlled in two ways: its enzymatic activity through protein-protein interactions and its transcription by transcriptional or posttranscriptional regulation.

KW - 14-3-3 protein

KW - Arabidopsis

KW - Ascorbate peroxidase

KW - Oxidative stress

KW - Peroxisome

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JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 6

ER -

Cloning and expression of an Arabidopsis gene encoding a putative peroxisomal ascorbate peroxidase

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