Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA

Cesar Llave; Zhixin Xie; Kristin D. Kasschau; James C. Carrington

doi:10.1126/science.1076311

Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA

Cesar Llave, Zhixin Xie, Kristin D. Kasschau, James C. Carrington

Biological Sciences

Research output: Contribution to journal › Article › peer-review

1351 Scopus citations

Abstract

Micro-RNAs (miRNAs) are regulatory molecules that mediate effects by interacting with messenger RNA (mRNA) targets. Here we show that Arabidopsis thaliana miRNA 39 (also known as miR171), a 21-ribonucleotide species that accumulates predominantly in inflorescence tissues, is produced from an intergenic region in chromosome III and functionally interacts with mRNA targets encoding several members of the Scarecrow-like (SCL) family of putative transcription factors. miRNA 39 is complementary to an internal region of three SCL mRNAs. The interaction results in specific cleavage of target mRNA within the region of complementarity, indicating that this class of miRNA functions like small interfering RNA associated with RNA silencing to guide sequence-specific cleavage in a developmentally controlled manner.

Original language	English
Pages (from-to)	2053-2056
Number of pages	4
Journal	Science
Volume	297
Issue number	5589
DOIs	https://doi.org/10.1126/science.1076311
State	Published - Sep 20 2002

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AB - Micro-RNAs (miRNAs) are regulatory molecules that mediate effects by interacting with messenger RNA (mRNA) targets. Here we show that Arabidopsis thaliana miRNA 39 (also known as miR171), a 21-ribonucleotide species that accumulates predominantly in inflorescence tissues, is produced from an intergenic region in chromosome III and functionally interacts with mRNA targets encoding several members of the Scarecrow-like (SCL) family of putative transcription factors. miRNA 39 is complementary to an internal region of three SCL mRNAs. The interaction results in specific cleavage of target mRNA within the region of complementarity, indicating that this class of miRNA functions like small interfering RNA associated with RNA silencing to guide sequence-specific cleavage in a developmentally controlled manner.

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Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA

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