TY - JOUR
T1 - Characterization of the long pentraxin PTX3 as a TNFα-induced secreted protein of adipose cells
AU - Abderrahim-Ferkoune, Anissa
AU - Bezy, Olivier
AU - Chiellini, Chiara
AU - Maffei, Margherita
AU - Grimaldi, Paul
AU - Bonino, Frédéric
AU - Moustaid-Moussa, Naïma
AU - Pasqualini, Fabio
AU - Mantovani, Alberto
AU - Ailhaud, Gérard
AU - Amri, Ez Zoubir
PY - 2003/5
Y1 - 2003/5
N2 - Exposure of preadipocytes to long-chain fatty acids induces the expression of several markers of adipocyte differentiation. In an attempt to identify novel genes and proteins that are regulated by fatty acids in preadipocytes, we performed a substractive hybridization screening and identified PTX3, a protein of the pentraxin family. PTX3 mRNA expression is transient during adipocyte differentiation of clonal cell lines and is absent in fully differentiated cells. Stable overexpression of PTX3 in preadipocytes has no effect on adipocyte differentiation. In line with this, PTX3 mRNA is expressed in the stromal-vascular fraction of adipose tissue, but not in the adipocyte fraction; however, in 3T3-F442A adipocytes, the PTX3 gene can be reinduced by tumor necrosis factor α (TNFα) in a dose-dependent manner. This effect is accompanied by PTX3 protein secretion from both 3T3-F442A adipocytes and explants of mouse adipose tissue. PTX3 mRNA levels are found to be higher in adipose tissue of genetically obese mice versus control mice, consistent with their increased TNFα levels. In conclusion, PTX3 appears as a TNFα-induced protein that provides a new link between chronic low-level inflammatory state and obesity.
AB - Exposure of preadipocytes to long-chain fatty acids induces the expression of several markers of adipocyte differentiation. In an attempt to identify novel genes and proteins that are regulated by fatty acids in preadipocytes, we performed a substractive hybridization screening and identified PTX3, a protein of the pentraxin family. PTX3 mRNA expression is transient during adipocyte differentiation of clonal cell lines and is absent in fully differentiated cells. Stable overexpression of PTX3 in preadipocytes has no effect on adipocyte differentiation. In line with this, PTX3 mRNA is expressed in the stromal-vascular fraction of adipose tissue, but not in the adipocyte fraction; however, in 3T3-F442A adipocytes, the PTX3 gene can be reinduced by tumor necrosis factor α (TNFα) in a dose-dependent manner. This effect is accompanied by PTX3 protein secretion from both 3T3-F442A adipocytes and explants of mouse adipose tissue. PTX3 mRNA levels are found to be higher in adipose tissue of genetically obese mice versus control mice, consistent with their increased TNFα levels. In conclusion, PTX3 appears as a TNFα-induced protein that provides a new link between chronic low-level inflammatory state and obesity.
KW - Adipocytes
KW - Cytokines
KW - Tumor necrosis factor α
UR - http://www.scopus.com/inward/record.url?scp=10744233013&partnerID=8YFLogxK
U2 - 10.1194/jlr.M200382-JLR200
DO - 10.1194/jlr.M200382-JLR200
M3 - Article
C2 - 12611905
AN - SCOPUS:10744233013
SN - 0022-2275
VL - 44
SP - 994
EP - 1000
JO - Journal of lipid research
JF - Journal of lipid research
IS - 5
ER -