TY - JOUR
T1 - Characterization of PDMS-modified glass from cast-and-peel fabrication
AU - Liu, Ke
AU - Tian, Yu
AU - Pitchimani, Rajesekar
AU - Huang, Michael
AU - Lincoln, Hawa
AU - Pappas, Dimitri
PY - 2009/7/15
Y1 - 2009/7/15
N2 - In glass/poly(dimethylsiloxane) (PDMS) hybrid microfluidic chips, two different fabrication approaches are used: photolithographic or solid ink molds, or cast-and-peel methods. In the latter, a thin slab of PDMS is laid down and fluid channels are cut manually or by machine. The cast-and-peel approach has been used successfully for low-shear culture devices, among other applications. The main drawback, not reported to date, of cast-and-peel methods is that removal of PDMS (exposing the glass substrate) results in nanoscopic domains of PDMS still attached to the surface. This residual PDMS is not observable by eye, but affects the hydrophobicity of the device. Using contact angle measurement, atomic force and fluorescence microscopy, the changes in glass surfaces from the cast-and-peel technique were elucidated. This study demonstrates the enhanced protein (NeutrAvidin) adsorption on PDMS treated glass surfaces, and the potential influence of altered glass properties on microfluidic applications has been discussed as well.
AB - In glass/poly(dimethylsiloxane) (PDMS) hybrid microfluidic chips, two different fabrication approaches are used: photolithographic or solid ink molds, or cast-and-peel methods. In the latter, a thin slab of PDMS is laid down and fluid channels are cut manually or by machine. The cast-and-peel approach has been used successfully for low-shear culture devices, among other applications. The main drawback, not reported to date, of cast-and-peel methods is that removal of PDMS (exposing the glass substrate) results in nanoscopic domains of PDMS still attached to the surface. This residual PDMS is not observable by eye, but affects the hydrophobicity of the device. Using contact angle measurement, atomic force and fluorescence microscopy, the changes in glass surfaces from the cast-and-peel technique were elucidated. This study demonstrates the enhanced protein (NeutrAvidin) adsorption on PDMS treated glass surfaces, and the potential influence of altered glass properties on microfluidic applications has been discussed as well.
KW - Atomic force microscopy
KW - Biotin-rhodamine 110
KW - Microfluidics
KW - NeutrAvidin
KW - PDMS
KW - Rhodamine B
UR - http://www.scopus.com/inward/record.url?scp=67549131987&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2009.03.058
DO - 10.1016/j.talanta.2009.03.058
M3 - Article
C2 - 19559887
AN - SCOPUS:67549131987
VL - 79
SP - 333
EP - 338
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 2
ER -