Characterization of lip expression in Salmonella typhimurium: Analysis of lip::lac operon fusions

R. L. Smith; J. W. Pelley; R. M. Jeter

doi:10.1099/00221287-137-10-2307

Characterization of lip expression in Salmonella typhimurium: Analysis of lip::lac operon fusions

R. L. Smith, J. W. Pelley, R. M. Jeter

Biological Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Strains of Salmonella typhimurium which have an auxotrophic requirement for lipoic acid were isolated by mutagenesis with the transposable element Mu dJ. The chromosomal location of these insertion mutations was determined to be at 14 map units by bacteriophage P22-mediated cotransduction. The lip gene is transcribed in the clockwise direction relative to the S. typhimurium genetic map. Strains with lip::lac operon fusions were used to characterize the transcriptional activity of the lip promoter. Transcription of the lip gene is not regulated by catabolite repression or lipoic acid concentration. The data indicate that the lip gene product is expressed constitutively at a low level.

Original language	English
Pages (from-to)	2307-2312
Number of pages	6
Journal	Journal of General Microbiology
Volume	137
Issue number	10
DOIs	https://doi.org/10.1099/00221287-137-10-2307
State	Published - 1991

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title = "Characterization of lip expression in Salmonella typhimurium: Analysis of lip::lac operon fusions",

abstract = "Strains of Salmonella typhimurium which have an auxotrophic requirement for lipoic acid were isolated by mutagenesis with the transposable element Mu dJ. The chromosomal location of these insertion mutations was determined to be at 14 map units by bacteriophage P22-mediated cotransduction. The lip gene is transcribed in the clockwise direction relative to the S. typhimurium genetic map. Strains with lip::lac operon fusions were used to characterize the transcriptional activity of the lip promoter. Transcription of the lip gene is not regulated by catabolite repression or lipoic acid concentration. The data indicate that the lip gene product is expressed constitutively at a low level.",

author = "Smith, {R. L.} and Pelley, {J. W.} and Jeter, {R. M.}",

year = "1991",

doi = "10.1099/00221287-137-10-2307",

language = "English",

volume = "137",

pages = "2307--2312",

journal = "Journal of General Microbiology",

issn = "0022-1287",

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T1 - Characterization of lip expression in Salmonella typhimurium: Analysis of lip::lac operon fusions

AU - Smith, R. L.

AU - Pelley, J. W.

AU - Jeter, R. M.

PY - 1991

Y1 - 1991

N2 - Strains of Salmonella typhimurium which have an auxotrophic requirement for lipoic acid were isolated by mutagenesis with the transposable element Mu dJ. The chromosomal location of these insertion mutations was determined to be at 14 map units by bacteriophage P22-mediated cotransduction. The lip gene is transcribed in the clockwise direction relative to the S. typhimurium genetic map. Strains with lip::lac operon fusions were used to characterize the transcriptional activity of the lip promoter. Transcription of the lip gene is not regulated by catabolite repression or lipoic acid concentration. The data indicate that the lip gene product is expressed constitutively at a low level.

AB - Strains of Salmonella typhimurium which have an auxotrophic requirement for lipoic acid were isolated by mutagenesis with the transposable element Mu dJ. The chromosomal location of these insertion mutations was determined to be at 14 map units by bacteriophage P22-mediated cotransduction. The lip gene is transcribed in the clockwise direction relative to the S. typhimurium genetic map. Strains with lip::lac operon fusions were used to characterize the transcriptional activity of the lip promoter. Transcription of the lip gene is not regulated by catabolite repression or lipoic acid concentration. The data indicate that the lip gene product is expressed constitutively at a low level.

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U2 - 10.1099/00221287-137-10-2307

DO - 10.1099/00221287-137-10-2307

M3 - Article

C2 - 1663151

AN - SCOPUS:0026002117

VL - 137

SP - 2307

EP - 2312

JO - Journal of General Microbiology

JF - Journal of General Microbiology

SN - 0022-1287

IS - 10

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