TY - JOUR
T1 - Characterization of jet injection efficiency with mouse cadavers
AU - Marston, Jeremy O.
AU - Lacerda, Carla M.R.
N1 - Funding Information:
This work was financially supported by The National Science Foundation via award number NSF-CBET-1749382 . The authors would like to thank the staff of the animal care services and Will Hauser for assistance with the experimental work, and Paul Fisher and Kate Broderick of Inovio Pharmaceuticals for helpful comments on the manuscript preparation. We also thank the anonymous reviewers, whose comments have greatly improved the discussion in this manuscript.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/7/10
Y1 - 2019/7/10
N2 - Needle-free drug delivery is highly sought after for reduction in sharps waste, prevention of needle-stick injuries, and potential for improved drug dispersion and uptake. Whilst there is a wealth of literature on the array of different delivery methods, jet injection is proposed as the sole candidate for delivery of viscous fluids, which is especially relevant with the advent of DNA-based vaccines. The focus of this study was therefore to assess the role of viscosity and jet configuration (i.e. stand-off relative to the skin)upon injection efficiency for a fixed spring-loaded system (Bioject ID Pen). We performed this assessment in the context of mouse cadavers and found that the dominant factor in determining success rates was the time from euthanasia, which was taken as a proxy for the stiffness of the underlying tissue. For overall injection efficiency, ANOVA tests indicated that stiffness was highly significant (P < < 0.001), stand-off was moderately significant (P < 0.1), and viscosity was insignificant. In contrast, both viscosity and standoff were found to be significant (P < 0.01)when evaluating the percentage delivered intradermally. Using high-resolution micro-computed tomography (μ-CT), we also determined the depth and overall dispersion pattern immediately after injection.
AB - Needle-free drug delivery is highly sought after for reduction in sharps waste, prevention of needle-stick injuries, and potential for improved drug dispersion and uptake. Whilst there is a wealth of literature on the array of different delivery methods, jet injection is proposed as the sole candidate for delivery of viscous fluids, which is especially relevant with the advent of DNA-based vaccines. The focus of this study was therefore to assess the role of viscosity and jet configuration (i.e. stand-off relative to the skin)upon injection efficiency for a fixed spring-loaded system (Bioject ID Pen). We performed this assessment in the context of mouse cadavers and found that the dominant factor in determining success rates was the time from euthanasia, which was taken as a proxy for the stiffness of the underlying tissue. For overall injection efficiency, ANOVA tests indicated that stiffness was highly significant (P < < 0.001), stand-off was moderately significant (P < 0.1), and viscosity was insignificant. In contrast, both viscosity and standoff were found to be significant (P < 0.01)when evaluating the percentage delivered intradermally. Using high-resolution micro-computed tomography (μ-CT), we also determined the depth and overall dispersion pattern immediately after injection.
KW - Intradermal
KW - Intramuscular
KW - Liquid jet
KW - Mouse cadaver
KW - Needle-free injection
UR - http://www.scopus.com/inward/record.url?scp=85066052011&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2019.05.023
DO - 10.1016/j.jconrel.2019.05.023
M3 - Article
C2 - 31112720
AN - SCOPUS:85066052011
VL - 305
SP - 101
EP - 109
JO - Journal of Controlled Release
JF - Journal of Controlled Release
SN - 0168-3659
ER -