Characterization of isomeric glycan structures by LC-MS/MS

Lucas Veillon, Yifan Huang, Wenjing Peng, Xue Dong, Byeong Gwan Cho, Yehia Mechref

Research output: Contribution to journalReview articlepeer-review

76 Scopus citations

Abstract

The characterization of glycosylation is critical for obtaining a comprehensive view of the regulation and functions of glycoproteins of interest. Due to the complex nature of oligosaccharides, stemming from variable compositions and linkages, and ion suppression effects, the chromatographic separation of glycans, including isomeric structures, is necessary for exhaustive characterization by MS. This review introduces the fundamental principles underlying the techniques in LC utilized by modern day glycomics researchers. Recent advances in porous graphitized carbon, reverse phase, ion exchange, and hydrophilic interaction LC utilized in conjunction with MS, for the characterization of protein glycosylation, are described with an emphasis on methods capable of resolving isomeric glycan structures.

Original languageEnglish
Pages (from-to)2100-2114
Number of pages15
JournalELECTROPHORESIS
Volume38
Issue number17
DOIs
StatePublished - Sep 2017

Keywords

  • Glycan analysis
  • Isomeric separation
  • LC-MS/MS
  • Liquid chromatography
  • Mass spectrometry

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