Characterization of a rice sucrose-phosphate synthase-encoding gene

Juan Jose Valdez-Alarcón; Marcelo Ferrando; Graciela Salerno; Beatriz Jimenez-Moraila; Luis Herrera-Estrella

doi:10.1016/0378-1119(95)00854-3

Characterization of a rice sucrose-phosphate synthase-encoding gene

Juan Jose Valdez-Alarcón, Marcelo Ferrando, Graciela Salerno, Beatriz Jimenez-Moraila, Luis Herrera-Estrella

Plant & Soil Science

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A rice genomic clone (sps1) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice sps1 contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice sps1 expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.

Original language	English
Pages (from-to)	217-222
Number of pages	6
Journal	Gene
Volume	170
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(95)00854-3
State	Published - May 8 1996

Keywords

Carbohydrate metabolism
Carbon partitioning
Sink-source interaction
Sucrose synthesis

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10.1016/0378-1119(95)00854-3

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@article{c119cb49585848d599bedc263e91048f,

title = "Characterization of a rice sucrose-phosphate synthase-encoding gene",

abstract = "A rice genomic clone (sps1) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice sps1 contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice sps1 expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.",

keywords = "Carbohydrate metabolism, Carbon partitioning, Sink-source interaction, Sucrose synthesis",

author = "Valdez-Alarc{\'o}n, {Juan Jose} and Marcelo Ferrando and Graciela Salerno and Beatriz Jimenez-Moraila and Luis Herrera-Estrella",

note = "Funding Information: This work supported in part by grants from the Howard Hughes Biomedical Institute (75191-526901) and the Rockefeller Foundation (90032-65) to L.H.-E. The kDASH library was a generous gift from S. Kay from Rockefeller University.",

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doi = "10.1016/0378-1119(95)00854-3",

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T1 - Characterization of a rice sucrose-phosphate synthase-encoding gene

AU - Valdez-Alarcón, Juan Jose

AU - Ferrando, Marcelo

AU - Salerno, Graciela

AU - Jimenez-Moraila, Beatriz

AU - Herrera-Estrella, Luis

N1 - Funding Information: This work supported in part by grants from the Howard Hughes Biomedical Institute (75191-526901) and the Rockefeller Foundation (90032-65) to L.H.-E. The kDASH library was a generous gift from S. Kay from Rockefeller University.

PY - 1996/5/8

Y1 - 1996/5/8

N2 - A rice genomic clone (sps1) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice sps1 contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice sps1 expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.

AB - A rice genomic clone (sps1) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice sps1 contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice sps1 expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.

KW - Carbohydrate metabolism

KW - Carbon partitioning

KW - Sink-source interaction

KW - Sucrose synthesis

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VL - 170

SP - 217

EP - 222

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -

Characterization of a rice sucrose-phosphate synthase-encoding gene

Abstract

Keywords

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