TY - JOUR
T1 - Characterization of a rice sucrose-phosphate synthase-encoding gene
AU - Valdez-Alarcón, Juan Jose
AU - Ferrando, Marcelo
AU - Salerno, Graciela
AU - Jimenez-Moraila, Beatriz
AU - Herrera-Estrella, Luis
N1 - Funding Information:
This work supported in part by grants from the Howard Hughes Biomedical Institute (75191-526901) and the Rockefeller Foundation (90032-65) to L.H.-E. The kDASH library was a generous gift from S. Kay from Rockefeller University.
PY - 1996/5/8
Y1 - 1996/5/8
N2 - A rice genomic clone (sps1) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice sps1 contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice sps1 expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.
AB - A rice genomic clone (sps1) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice sps1 contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice sps1 expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.
KW - Carbohydrate metabolism
KW - Carbon partitioning
KW - Sink-source interaction
KW - Sucrose synthesis
UR - http://www.scopus.com/inward/record.url?scp=0030000775&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(95)00854-3
DO - 10.1016/0378-1119(95)00854-3
M3 - Article
C2 - 8666248
AN - SCOPUS:0030000775
VL - 170
SP - 217
EP - 222
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -