Characterization and transferability of class 1 integrons in commensal bacteria isolated from farm and nonfarm environments

Hua Yang, Oleksandr A. Byelashov, Ifigenia Geornaras, Lawrence D. Goodridge, Kendra K. Nightingale, Keith E. Belk, Gary C. Smith, John N. Sofos

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

This study assessed the distribution of class 1 integrons in commensal bacteria isolated from agricultural and nonfarm environments, and the transferability of class 1 integrons to pathogenic bacteria. A total of 26 class 1 integron-positive isolates were detected in fecal samples from cattle operations and a city park, water samples from a beef ranch and city lakes, and soil, feed (unused), manure, and compost samples from a dairy farm. Antimicrobial susceptibility testing of class 1 integron-positive Enterobacteriaceae isolates from city locations displayed multi-resistance to 12-13 out of the 22 antibiotics tested, whereas class 1 integron-positive Enterobacteriaceae isolates from cattle operations only displayed tetracycline resistance. Most class 1 integrons had one gene cassette belonging to the aadA family that confers resistance to streptomycin and spectinomycin. One isolate from a dog fecal sample collected from a city dog park transferred its class 1 integron to a strain of Escherichia coli O157:H7 at a frequency of 10 -7 transconjugants/donor by in vitro filter mating experiments under the stated laboratory conditions. Due to the numerous factors that may affect the transferability testing, further investigation using different methodologies may be helpful to reveal the transferability of the integrons from other isolates. The presence of class 1 integrons among diverse commensal bacteria from agricultural and nonfarm environments strengthens the possible role of environmental commensals in serving as reservoirs of antibiotic resistance genes.

Original languageEnglish
Pages (from-to)1441-1451
Number of pages11
JournalFoodborne Pathogens and Disease
Volume7
Issue number12
DOIs
StatePublished - Dec 1 2010

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