TY - JOUR
T1 - Cellular separations
T2 - A review of new challenges in analytical chemistry
AU - Pappas, Dimitri
AU - Wang, Kelong
PY - 2007/10/1
Y1 - 2007/10/1
N2 - The ability to generate a sample of cells of a given phenotype is a prerequisite for many cellular assays. In response to this growing need, numerous methods for cell separation have been developed in recent years. This Review covers recent progress in the field of cell separations and cell chromatography. Cell separation principles-such as size and affinity capture-are discussed, as well as conventional methods such as fluorescence-activated cell sorting and magnetic sorting. Planar flow cell arrays, dielectrophoresis, field-flow methods, and column separation devices are reviewed, as well as applications of these methods to medicine and biotechnology. Cell attachment and adhesion strategies and a comparison of techniques are also presented.
AB - The ability to generate a sample of cells of a given phenotype is a prerequisite for many cellular assays. In response to this growing need, numerous methods for cell separation have been developed in recent years. This Review covers recent progress in the field of cell separations and cell chromatography. Cell separation principles-such as size and affinity capture-are discussed, as well as conventional methods such as fluorescence-activated cell sorting and magnetic sorting. Planar flow cell arrays, dielectrophoresis, field-flow methods, and column separation devices are reviewed, as well as applications of these methods to medicine and biotechnology. Cell attachment and adhesion strategies and a comparison of techniques are also presented.
KW - Affinity chromatography
KW - Cell chromatography
KW - Cell separation
KW - Cell sorting
KW - Cytometry
UR - http://www.scopus.com/inward/record.url?scp=34548841925&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2007.08.033
DO - 10.1016/j.aca.2007.08.033
M3 - Review article
C2 - 17904469
AN - SCOPUS:34548841925
VL - 601
SP - 26
EP - 35
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1
ER -