Cell culture chip using low-shear mass transport

Ke Liu, Rajasekar Pitchimani, Dana Dang, Keith Bayer, Tyler Harrington, Dimitri Pappas

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

We have developed a flow cell that allows culturing adherent cells as well as suspended cells in a stable, homogeneous, and low-shear force environment. The device features continuous medium supply and waste exchange. In this paper, a simple and fast protocol for device design, fabrication, and assembly (sealing) based on a poly(dimethylsiloxane) (PMDS)/glass slide hybrid structure is described. The cell culture system performance was monitored, and the effective shear force inside the culture well was also determined. By manipulating the device dimensions and volumetric flow rate, shear stress was controlled during experiments. Cell adhesion, growth, proliferation, and death over long-term culture periods were observed by microscopy. The growth of both endothelial and suspension cells in this device exhibited comparable characteristics to those of traditional approaches. The low-shear culture device significantly reduced shear stress encountered in microfluidic systems, allowing both adherent and suspended cells to be grown in a simple device.

Original languageEnglish
Pages (from-to)5955-5960
Number of pages6
JournalLangmuir
Volume24
Issue number11
DOIs
StatePublished - Jun 3 2008

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    Liu, K., Pitchimani, R., Dang, D., Bayer, K., Harrington, T., & Pappas, D. (2008). Cell culture chip using low-shear mass transport. Langmuir, 24(11), 5955-5960. https://doi.org/10.1021/la8003917