Cell affinity separations on microfluidic devices

Yan Gao; Wenjie Li; Ye Zhang; Dimitri Pappas

doi:10.1007/978-1-4939-2447-9_6

Cell affinity separations on microfluidic devices

Yan Gao, Wenjie Li, Ye Zhang, Dimitri Pappas

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Separating cells from a heterogeneous sample on microfluidic devices is a very important unit operation in biological and medical research. Microfluidic affinity cell chromatography is a label-free separation technique, providing ease of operation, low cost, and rapid analysis. In this chapter, protocols for cell affinity separation in polydimethylsiloxane (PDMS)–glass microdevices and glass capillaries are described.

Original language	English
Pages (from-to)	55-65
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1286
DOIs	https://doi.org/10.1007/978-1-4939-2447-9_6
State	Published - 2015

Keywords

Affinity chromatography
Cell separation
Glass capillaries
Microfluidic device
Polydimethylsiloxane

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10.1007/978-1-4939-2447-9_6

Cite this

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keywords = "Affinity chromatography, Cell separation, Glass capillaries, Microfluidic device, Polydimethylsiloxane",

author = "Yan Gao and Wenjie Li and Ye Zhang and Dimitri Pappas",

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AU - Li, Wenjie

AU - Zhang, Ye

AU - Pappas, Dimitri

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PY - 2015

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AB - Separating cells from a heterogeneous sample on microfluidic devices is a very important unit operation in biological and medical research. Microfluidic affinity cell chromatography is a label-free separation technique, providing ease of operation, low cost, and rapid analysis. In this chapter, protocols for cell affinity separation in polydimethylsiloxane (PDMS)–glass microdevices and glass capillaries are described.

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KW - Cell separation

KW - Glass capillaries

KW - Microfluidic device

KW - Polydimethylsiloxane

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Cell affinity separations on microfluidic devices

Abstract

Keywords

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