TY - JOUR
T1 - Carbon isotopic fractionations associated with thermophilic bacteria Thermotoga maritima and Persephonella marina
AU - Zhang, Chuanlun L.
AU - Ye, Qi
AU - Reysenbach, Anna Louise
AU - Götz, Dorothee
AU - Peacock, Aaron
AU - White, David C.
AU - Horita, Juske
AU - Cole, David R.
AU - Fong, Jon
AU - Pratt, Lisa
AU - Fang, Jiasong
AU - Huang, Yongsong
PY - 2002
Y1 - 2002
N2 - Stable carbon isotopes can provide insight into carbon cycling pathways in natural environments. We examined carbon isotope fractionations associated with a hyperthermophilic fermentative bacterium, Thermotoga maritima, and a thermophilic chemolithoautotrophic bacterium Persephonella marina. In T. maritima, phospholipid fatty acids (PLFA) are slightly enriched in 13C relative to biomass (ε =0.1-0.8‰). However, PLFA and biomass are depleted in 13C relative to the substrate glucose by ∼8‰. In P. marina, PLFA are 1.8-14.5‰ enriched in 13C relative to biomass, which suggests that the reversed tricarboxylic acid (TCA) cycle or the 3-hydroxypropionate pathway may be used for CO2 fixation. This is supported by small fractionation between biomass and CO2 (ε = -3.8‰ to -5.0‰), which is similar to fractionations reported for other organisms using Similar CO2 fixation pathways. Identification of the exact pathway will require biochemical assay for specific enzymes associated with the reversed TCA cycle or the 3-hydroxypropionate pathway.
AB - Stable carbon isotopes can provide insight into carbon cycling pathways in natural environments. We examined carbon isotope fractionations associated with a hyperthermophilic fermentative bacterium, Thermotoga maritima, and a thermophilic chemolithoautotrophic bacterium Persephonella marina. In T. maritima, phospholipid fatty acids (PLFA) are slightly enriched in 13C relative to biomass (ε =0.1-0.8‰). However, PLFA and biomass are depleted in 13C relative to the substrate glucose by ∼8‰. In P. marina, PLFA are 1.8-14.5‰ enriched in 13C relative to biomass, which suggests that the reversed tricarboxylic acid (TCA) cycle or the 3-hydroxypropionate pathway may be used for CO2 fixation. This is supported by small fractionation between biomass and CO2 (ε = -3.8‰ to -5.0‰), which is similar to fractionations reported for other organisms using Similar CO2 fixation pathways. Identification of the exact pathway will require biochemical assay for specific enzymes associated with the reversed TCA cycle or the 3-hydroxypropionate pathway.
UR - http://www.scopus.com/inward/record.url?scp=0036009693&partnerID=8YFLogxK
U2 - 10.1046/j.1462-2920.2002.00266.x
DO - 10.1046/j.1462-2920.2002.00266.x
M3 - Article
C2 - 11966826
AN - SCOPUS:0036009693
SN - 1462-2912
VL - 4
SP - 58
EP - 64
JO - Environmental Microbiology
JF - Environmental Microbiology
IS - 1
ER -