TY - JOUR
T1 - Capillary electrophoresis of herbicides. 1. Precolumn derivatization of chiral and achiral phenoxy acid herbicides with a fluorescent tag for electrophoretic separation in the presence of cyclodextrins and micellar phases
AU - Mechref, Yehia
AU - Rassi, Ziad El
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1996
Y1 - 1996
N2 - A novel, selective precolumn derivatization reaction was introduced and evaluated in the fluorescence labeling of phenoxy acid herbicides with 7-aminonaphthalene-1,3-disulfonic acid (ANDSA). The ANDSA-phenoxy acid derivatives were readily detected by capillary electrophoresis/laser-induced fluorescence (CE-LIF) at 0.2 ppb, a concentration at which environmental herbicide samples are expected to occur. The precolumn derivatization was very quantitative (99.7% yield) and produced stable derivatives and no side products. Furthermore, the ANDSA-phenoxy acid herbicide enantiomers exhibited higher chiral resolution than their underivatized counter-parts hi the presence of cyclodextrin (CD) hi the running electrolyte. Several native and modified CDs were investigated in the enantiomeric separation of chiral phenoxy acid herbicides. The best enantioselectivity was achieved when 2,3,6-tri-O-methyl-β-cyclodextrin (TM-β-CD) was used as the choral selector. To effect the enantiomeric resolution of a large number of ANDSA-phenoxy acid enantiomers, electrolyte systems based on mixed CDs were utilized and evaluated. Mixed CDs based on β-CD and TM-β-CD proved to be the most effective as far as the enantiomeric resolution of the chiral anahtes is concerned. To provide different selectivity for the various ANDSA-phenoxy acid herbicides, novel micellar systems containing cyclodextrins were evaluated.
AB - A novel, selective precolumn derivatization reaction was introduced and evaluated in the fluorescence labeling of phenoxy acid herbicides with 7-aminonaphthalene-1,3-disulfonic acid (ANDSA). The ANDSA-phenoxy acid derivatives were readily detected by capillary electrophoresis/laser-induced fluorescence (CE-LIF) at 0.2 ppb, a concentration at which environmental herbicide samples are expected to occur. The precolumn derivatization was very quantitative (99.7% yield) and produced stable derivatives and no side products. Furthermore, the ANDSA-phenoxy acid herbicide enantiomers exhibited higher chiral resolution than their underivatized counter-parts hi the presence of cyclodextrin (CD) hi the running electrolyte. Several native and modified CDs were investigated in the enantiomeric separation of chiral phenoxy acid herbicides. The best enantioselectivity was achieved when 2,3,6-tri-O-methyl-β-cyclodextrin (TM-β-CD) was used as the choral selector. To effect the enantiomeric resolution of a large number of ANDSA-phenoxy acid enantiomers, electrolyte systems based on mixed CDs were utilized and evaluated. Mixed CDs based on β-CD and TM-β-CD proved to be the most effective as far as the enantiomeric resolution of the chiral anahtes is concerned. To provide different selectivity for the various ANDSA-phenoxy acid herbicides, novel micellar systems containing cyclodextrins were evaluated.
UR - http://www.scopus.com/inward/record.url?scp=0030145274&partnerID=8YFLogxK
U2 - 10.1021/ac951260f
DO - 10.1021/ac951260f
M3 - Article
AN - SCOPUS:0030145274
VL - 68
SP - 1771
EP - 1777
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 10
ER -