The determination of spermatozoal concentration is required for the accurate calculation of a breeding dose and subsequent dilution of semen for cooled semen transport. Sperm concentration should also be determined as part of a routine stallion breeding soundness examination. Calibrated spectrophotometers dedicated to estimating stallion sperm concentration are commercially available. This chapter discusses the step by step procedure for evaluation of the sperm concentration using a photometer and a spectrophotometer with illustrations, and the equipment and supplies needed. The chapter outlines the necessary conditions for the calibrated spectrophotometer evaluation of sperm concentration.
- Calibrated spectrophotometer evaluation
- Sperm concentration