Calibrated Spectrophotometer Evaluation of Sperm Concentration

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


The determination of spermatozoal concentration is required for the accurate calculation of a breeding dose and subsequent dilution of semen for cooled semen transport. Sperm concentration should also be determined as part of a routine stallion breeding soundness examination. Calibrated spectrophotometers dedicated to estimating stallion sperm concentration are commercially available. This chapter discusses the step by step procedure for evaluation of the sperm concentration using a photometer and a spectrophotometer with illustrations, and the equipment and supplies needed. The chapter outlines the necessary conditions for the calibrated spectrophotometer evaluation of sperm concentration.

Original languageEnglish
Title of host publicationEquine Reproductive Procedures
Number of pages3
ISBN (Electronic)9781118904398
ISBN (Print)9780470960394
StatePublished - Aug 25 2014


  • Calibrated spectrophotometer evaluation
  • Photometer
  • Sperm concentration
  • Stallion


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