A mutant (erg6) of Saccharomyces cerevisiae defective in S-adenosylmethionine: Δ24-sterol-C-methyl transferase (EC188.8.131.52) which normally produces cholesta-5,7,24-trienol and cholesta-5,7,22,24-tetraenol as the major sterols (total 4,4-desmethyl sterol content-8.3fg/cell) was shown to synthesize trace levels of cholesterol (0.08fg/cell). The identity of cholesterol was established by co-chromatography in TLC, GLC and HPLC with an authentic sample, mass spectroscopy and after an incubation with [1-14C]acetate by isotopic dilution and recrystallization of the radiochemically purified material to constant specific activity.
|Number of pages||9|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 30 1988|