TY - JOUR
T1 - Biosynthesis of cholesterol in the yeast mutant ERG6
AU - Sihua, Xu
AU - David Nes, W.
PY - 1988/8/30
Y1 - 1988/8/30
N2 - A mutant (erg6) of Saccharomyces cerevisiae defective in S-adenosylmethionine: Δ24-sterol-C-methyl transferase (EC2.1.1.41) which normally produces cholesta-5,7,24-trienol and cholesta-5,7,22,24-tetraenol as the major sterols (total 4,4-desmethyl sterol content-8.3fg/cell) was shown to synthesize trace levels of cholesterol (0.08fg/cell). The identity of cholesterol was established by co-chromatography in TLC, GLC and HPLC with an authentic sample, mass spectroscopy and after an incubation with [1-14C]acetate by isotopic dilution and recrystallization of the radiochemically purified material to constant specific activity.
AB - A mutant (erg6) of Saccharomyces cerevisiae defective in S-adenosylmethionine: Δ24-sterol-C-methyl transferase (EC2.1.1.41) which normally produces cholesta-5,7,24-trienol and cholesta-5,7,22,24-tetraenol as the major sterols (total 4,4-desmethyl sterol content-8.3fg/cell) was shown to synthesize trace levels of cholesterol (0.08fg/cell). The identity of cholesterol was established by co-chromatography in TLC, GLC and HPLC with an authentic sample, mass spectroscopy and after an incubation with [1-14C]acetate by isotopic dilution and recrystallization of the radiochemically purified material to constant specific activity.
UR - http://www.scopus.com/inward/record.url?scp=0023681436&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(88)81116-1
DO - 10.1016/S0006-291X(88)81116-1
M3 - Article
C2 - 3046617
AN - SCOPUS:0023681436
SN - 0006-291X
VL - 155
SP - 509
EP - 517
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -