Biosynthesis of cholesterol in the yeast mutant ERG6

Xu Sihua, W. David Nes

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A mutant (erg6) of Saccharomyces cerevisiae defective in S-adenosylmethionine: Δ24-sterol-C-methyl transferase (EC2.1.1.41) which normally produces cholesta-5,7,24-trienol and cholesta-5,7,22,24-tetraenol as the major sterols (total 4,4-desmethyl sterol content-8.3fg/cell) was shown to synthesize trace levels of cholesterol (0.08fg/cell). The identity of cholesterol was established by co-chromatography in TLC, GLC and HPLC with an authentic sample, mass spectroscopy and after an incubation with [1-14C]acetate by isotopic dilution and recrystallization of the radiochemically purified material to constant specific activity.

Original languageEnglish
Pages (from-to)509-517
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume155
Issue number1
DOIs
StatePublished - Aug 30 1988

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