TY - JOUR
T1 - Biolistic transformation of Mucor circinelloides
AU - González-Hernández, G. A.
AU - Herrera-Estrella, L.
AU - Rocha-Ramírez, V.
AU - Roncero, M. I.G.
AU - Gutierrez-Corona, J. F.
N1 - Funding Information:
We are grateful to Drs E. Cerda! -Olmedo and A. Herrera-Estrella for critical reading of the manuscript and Dr R. Zazueta-Sandoval for preparation of the photographs. This work was supported by the Consejo Nacional de Ciencia y Tecnologı!a, Me!xico.
PY - 1997
Y1 - 1997
N2 - Intact ungerminated spores or germinating swollen spores of Mucor circinelloides were biolistically transformed to leucine prototrophy with the autonomous replicating plasmids pLeu4 and pMCL1302. The former developmental stage was efficiently transformed with both plasmids. The existence of pLeu4 sequences, as selfreplicating elements, within Leu+ transformants was established by Southern analysis and by the recovery of plasmid pLeu4 in bacteria transformed with total DNA from M. circinelloides transformants. This is the first report of the transformation of intact cells of a member of the Zygomycetes.
AB - Intact ungerminated spores or germinating swollen spores of Mucor circinelloides were biolistically transformed to leucine prototrophy with the autonomous replicating plasmids pLeu4 and pMCL1302. The former developmental stage was efficiently transformed with both plasmids. The existence of pLeu4 sequences, as selfreplicating elements, within Leu+ transformants was established by Southern analysis and by the recovery of plasmid pLeu4 in bacteria transformed with total DNA from M. circinelloides transformants. This is the first report of the transformation of intact cells of a member of the Zygomycetes.
UR - http://www.scopus.com/inward/record.url?scp=2642704195&partnerID=8YFLogxK
U2 - 10.1017/S0953756297003614
DO - 10.1017/S0953756297003614
M3 - Article
AN - SCOPUS:2642704195
VL - 101
SP - 953
EP - 956
JO - Mycological Research
JF - Mycological Research
SN - 0953-7562
IS - 8
ER -