Biolistic transformation of Mucor circinelloides

G. A. González-Hernández; L. Herrera-Estrella; V. Rocha-Ramírez; M. I.G. Roncero; J. F. Gutierrez-Corona

doi:10.1017/S0953756297003614

Biolistic transformation of Mucor circinelloides

G. A. González-Hernández, L. Herrera-Estrella, V. Rocha-Ramírez, M. I.G. Roncero, J. F. Gutierrez-Corona

Plant & Soil Science

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Intact ungerminated spores or germinating swollen spores of Mucor circinelloides were biolistically transformed to leucine prototrophy with the autonomous replicating plasmids pLeu4 and pMCL1302. The former developmental stage was efficiently transformed with both plasmids. The existence of pLeu4 sequences, as selfreplicating elements, within Leu+ transformants was established by Southern analysis and by the recovery of plasmid pLeu4 in bacteria transformed with total DNA from M. circinelloides transformants. This is the first report of the transformation of intact cells of a member of the Zygomycetes.

Original language	English
Pages (from-to)	953-956
Number of pages	4
Journal	Mycological Research
Volume	101
Issue number	8
DOIs	https://doi.org/10.1017/S0953756297003614
State	Published - 1997

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title = "Biolistic transformation of Mucor circinelloides",

abstract = "Intact ungerminated spores or germinating swollen spores of Mucor circinelloides were biolistically transformed to leucine prototrophy with the autonomous replicating plasmids pLeu4 and pMCL1302. The former developmental stage was efficiently transformed with both plasmids. The existence of pLeu4 sequences, as selfreplicating elements, within Leu+ transformants was established by Southern analysis and by the recovery of plasmid pLeu4 in bacteria transformed with total DNA from M. circinelloides transformants. This is the first report of the transformation of intact cells of a member of the Zygomycetes.",

author = "Gonz{\'a}lez-Hern{\'a}ndez, {G. A.} and L. Herrera-Estrella and V. Rocha-Ram{\'i}rez and Roncero, {M. I.G.} and Gutierrez-Corona, {J. F.}",

year = "1997",

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language = "English",

volume = "101",

pages = "953--956",

journal = "Mycological Research",

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T1 - Biolistic transformation of Mucor circinelloides

AU - González-Hernández, G. A.

AU - Herrera-Estrella, L.

AU - Rocha-Ramírez, V.

AU - Roncero, M. I.G.

AU - Gutierrez-Corona, J. F.

PY - 1997

Y1 - 1997

N2 - Intact ungerminated spores or germinating swollen spores of Mucor circinelloides were biolistically transformed to leucine prototrophy with the autonomous replicating plasmids pLeu4 and pMCL1302. The former developmental stage was efficiently transformed with both plasmids. The existence of pLeu4 sequences, as selfreplicating elements, within Leu+ transformants was established by Southern analysis and by the recovery of plasmid pLeu4 in bacteria transformed with total DNA from M. circinelloides transformants. This is the first report of the transformation of intact cells of a member of the Zygomycetes.

AB - Intact ungerminated spores or germinating swollen spores of Mucor circinelloides were biolistically transformed to leucine prototrophy with the autonomous replicating plasmids pLeu4 and pMCL1302. The former developmental stage was efficiently transformed with both plasmids. The existence of pLeu4 sequences, as selfreplicating elements, within Leu+ transformants was established by Southern analysis and by the recovery of plasmid pLeu4 in bacteria transformed with total DNA from M. circinelloides transformants. This is the first report of the transformation of intact cells of a member of the Zygomycetes.

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JO - Mycological Research

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