Biochemical individuality reflected in chromatographic, electrophoretic and mass-spectrometric profiles

Milos V. Novotny, Helena A. Soini, Yehia Mechref

Research output: Contribution to journalReview articlepeer-review

35 Scopus citations

Abstract

This review discusses the current trends in molecular profiling for the emerging systems biology applications. Historically, the methodological developments in separation science were coincident with the availability of new ionization techniques in mass spectrometry. Coupling miniaturized separation techniques with technologically-advanced MS instrumentation and the modern data processing capabilities are at the heart of current platforms for proteomics, glycomics and metabolomics. These are being featured here by the examples from quantitative proteomics, glycan mapping and metabolomic profiling of physiological fluids.

Original languageEnglish
Pages (from-to)26-47
Number of pages22
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume866
Issue number1-2
DOIs
StatePublished - Apr 15 2008

Keywords

  • Animal models
  • Biodiversity
  • Bioinformatics
  • GC-MS
  • Glycomic profiling
  • LC-MS
  • Metabolic stable-isotope labeling
  • Metabolomic profiling
  • Quantitative proteomics
  • Stable-isotope labeling
  • Systems biology

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