Batrachochytrium dendrobatidis can infect and cause mortality in the nematode Caenorhabditis elegans.

E. J. Shapard, A. S. Moss, M. J. San Francisco

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Batrachochytrium dendrobatidis (Bd) has been identified as a causative agent in the precipitous decline of amphibians worldwide. Studies on the fungus including its ability to infect and kill the host require use of frogs, a precious resource. Therefore, the development of an alternate host model to study the virulence of the fungus would be useful. Here, we show that Bd can cause mortality in the nematode Caenorhabditis elegans. Incubation of Bd with C. elegans resulted in greater than 70% mortality in the nematodes over a period of 24 h. Fluorescence microscopy using propidium iodide, a fluorescent dye used to determine cell viability, and tactile assays were used to discriminate between live and dead nematodes. These observations suggest that C. elegans may be a useful model organism to study the pathogenicity and virulence mechanisms of Bd.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalMycopathologia
Volume173
Issue number2-3
DOIs
StatePublished - Mar 2012

Fingerprint Dive into the research topics of 'Batrachochytrium dendrobatidis can infect and cause mortality in the nematode Caenorhabditis elegans.'. Together they form a unique fingerprint.

Cite this