TY - JOUR
T1 - Bacteria-derived diacetyl enhances Arabidopsis phosphate starvation responses partially through the DELLA-dependent gibberellin signaling pathway
AU - Morcillo, Rafael J.L.
AU - Singh, Sunil K.
AU - He, Danxia
AU - Vílchez, Juan I.
AU - Kaushal, Richa
AU - Wang, Wei
AU - Huang, Weichang
AU - Paré, Paul W.
AU - Zhang, Huiming
N1 - Funding Information:
We thank Prof. Choong-Min Ryu (Korea Research Institute of Bioscience & Biotechnology, Korea) for B. amyloliquefaciens GB03, Prof. Nicholas P. Harberd (University of Oxford, UK) for ΔDELLA and pRGA:GFP-RGA. Research in H.Z. Lab has been supported by the Chinese Academy of Sciences (CAS) and by the Thousand Talents Program for Young Scientists, China. R.J.L.M. was supported by the CAS PIFI fellowship.
Publisher Copyright:
© 2020, © 2020 Taylor & Francis Group, LLC.
PY - 2020/4/2
Y1 - 2020/4/2
N2 - Plant growth-promoting rhizobacteria (PGPR) are naturally occurring soil microorganisms that colonize roots and stimulate plant growth. Some PGPR strains can directly regulate plant growth in the absence of physical contact with the plant, via volatile organic compounds (VOCs) emissions. Recently, we have described that Arabidopsis thaliana respond differentially to diacetyl, a VOC from Bacillus amyloliquefaciens strain GB03 (GB03), through integral modulation of the immune system and the phosphate-starvation response (PSR) system, resulting in either mutualism or immunity. Under phosphate deficient conditions, diacetyl enhances salicylic acid- and jasmonic acid-mediated immunity and consequently causes plant hyper-sensitivity to phosphate deficiency. Here, we show that application of exogenous gibberellin (GA) partially alleviates the deleterious effect caused by either B. amyloliquefaciens GB03 VOCs or diacetyl in Arabidopsis under phosphate deficient conditions, while DELLA quadruple mutant exposed to GB03 VOCs exhibits a partial reduction on the stress symptoms. Moreover, diacetyl appears to enhance DELLA protein accumulation and increase the expression of several GA deactivation-related genes. These findings suggest that the DELLA-mediated GA signaling pathway is involved in the bi-faceted role of GB03 VOCs in regulating plant growth.
AB - Plant growth-promoting rhizobacteria (PGPR) are naturally occurring soil microorganisms that colonize roots and stimulate plant growth. Some PGPR strains can directly regulate plant growth in the absence of physical contact with the plant, via volatile organic compounds (VOCs) emissions. Recently, we have described that Arabidopsis thaliana respond differentially to diacetyl, a VOC from Bacillus amyloliquefaciens strain GB03 (GB03), through integral modulation of the immune system and the phosphate-starvation response (PSR) system, resulting in either mutualism or immunity. Under phosphate deficient conditions, diacetyl enhances salicylic acid- and jasmonic acid-mediated immunity and consequently causes plant hyper-sensitivity to phosphate deficiency. Here, we show that application of exogenous gibberellin (GA) partially alleviates the deleterious effect caused by either B. amyloliquefaciens GB03 VOCs or diacetyl in Arabidopsis under phosphate deficient conditions, while DELLA quadruple mutant exposed to GB03 VOCs exhibits a partial reduction on the stress symptoms. Moreover, diacetyl appears to enhance DELLA protein accumulation and increase the expression of several GA deactivation-related genes. These findings suggest that the DELLA-mediated GA signaling pathway is involved in the bi-faceted role of GB03 VOCs in regulating plant growth.
KW - Diacetyl
KW - gibberellin
KW - immunity
KW - mutualism
KW - phosphate
KW - plant-bacteria interaction
UR - http://www.scopus.com/inward/record.url?scp=85082321060&partnerID=8YFLogxK
U2 - 10.1080/15592324.2020.1740872
DO - 10.1080/15592324.2020.1740872
M3 - Article
C2 - 32183580
AN - SCOPUS:85082321060
VL - 15
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
SN - 1559-2316
IS - 4
M1 - 1740872
ER -