Bacillus subtilis PB6 Supplementation in Weaned Holstein Steers during an Experimental Salmonella Challenge

Paul R. Broadway, Jeffery A. Carroll, Nicole C. Burdick Sanchez, Todd R. Callaway, Sara D. Lawhon, Elana V. Gart, Laura K. Bryan, David J. Nisbet, Heather D. Hughes, Jerrad F. Legako, Debra L. O'Connor, Jerilyn E. Hergenreder, P. Whitney Rounds

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


To evaluate the effects of a patented Bacillus subtilis probiotic, weaned Holstein steers, not shedding Salmonella (n = 40; ∼90 kg), were supplemented (CLO) or not (CON) with CLOSTAT® (13 g/hd per day; Kemin Industries, Des Moines, IA) in a starter ration for 35 d. The calves were assigned to one of four treatments in a 2 × 2 factorial design with CLO and CON calves that were orally administered Salmonella (STM) or not (NoSTM). Calves were challenged with 1.6 × 10colony-forming unit (CFU) Salmonella Typhimurium (resistant to 50 μg/mL nalidixic acid) in 1 L of milk replacer on day 0. Blood samples were collected through jugular catheters every 6 h for 96 h, and body temperature was measured every 5 min through indwelling rectal temperature recording devices. Five calves from each treatment were harvested 48 h postchallenge, and the remaining calves were harvested 96 h postchallenge. During necropsy, tissues were collected for the isolation and quantification of the inoculated STM from various tissues. The CLOSTM group had reduced STM concentrations in the jejunum, ileum, and transverse colon 48 h after the challenge (p ≤ 0.03), but were not different 96 h postchallenge (p > 0.05). Decreased (p < 0.01) pyrexia was observed after the challenge in CLOSTM calves when compared with CONSTM calves. White blood cells and lymphocyte counts were increased (p ≤ 0.05) in CLOSTM calves after the challenge in comparison with other treatments. In calves given STM, the CLO group had greater feed intake before and after the challenge (p < 0.01) compared with the CON group. Increased serum IL-6 and IFN-γconcentrations were observed in the CONSTM group compared with other treatments. Overall, CLO reduced Salmonella presence and concentrations in gastrointestinal tissues while simultaneously reducing the severity of the challenge as indicated by blood parameters and the reduced febrile response.

Original languageEnglish
Pages (from-to)521-528
Number of pages8
JournalFoodborne Pathogens and Disease
Issue number8
StatePublished - Aug 1 2020


  • Bacillus subtilis
  • Salmonella
  • calves
  • probiotic


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