TY - JOUR
T1 - Bacillus coahuilensis sp. nov., a moderately halophilic species from a desiccation lagoon in the Cuatro Ciénegas Valley in Coahuila, Mexico
AU - Cerritos, René
AU - Vinuesa, Pablo
AU - Eguiarte, Luis E.
AU - Herrera-Estrella, Luis
AU - Alcaraz-Peraza, Luis D.
AU - Arvizu-Gómez, Jackeline L.
AU - Olmedo, Gabriela
AU - Ramirez, Enrique
AU - Siefert, Janet L.
AU - Souza, Valeria
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2008/4
Y1 - 2008/4
N2 - A moderately halophilic, Gram-positive and rod-shaped bacterium, strain m4-4T, Was isolated from a Chihuahuan desert lagoon in Cuatro Ciénegas, Coahuila, Mexico. Strain m4-4T was found to grow optimally at 30-37 °C, pH 7.0-8.0 and 5 % NaCl and to tolerate from 0.5 % to 10 % NaCl. It was shown to be aerobic. The genomic DNA G + Ccontent was about 37 mol%. Strain m4-4T exhibited minimal or no growth on most sugars tested. Its major cellular fatty acids were C14:0, C16:0 and C18:1. Based on phylogenetic analysis of 16S rRNA and recA gene sequences, we observed that the closest relatives of the isolate are moderately halophilic Bacillus species, with 16S rRNA gene sequence similarity ranging from 96.6 to 97.4% (Bacillus marisflavi, Bacillus aquimaris and Bacillus vietnamensis). Additionally, using genomic data it was determined that the type strain contains a total of nine rRNA operons with three slightly different sequences. On the basis of phenotypic and molecular properties, strain m4-4T represents a hovel species within the genus Bacillus, for which the name Bacillus coahuilensis sp. no,v. is proposed, with the type strain m4-4T (=NRRL B-41737T =CECT 7197T).
AB - A moderately halophilic, Gram-positive and rod-shaped bacterium, strain m4-4T, Was isolated from a Chihuahuan desert lagoon in Cuatro Ciénegas, Coahuila, Mexico. Strain m4-4T was found to grow optimally at 30-37 °C, pH 7.0-8.0 and 5 % NaCl and to tolerate from 0.5 % to 10 % NaCl. It was shown to be aerobic. The genomic DNA G + Ccontent was about 37 mol%. Strain m4-4T exhibited minimal or no growth on most sugars tested. Its major cellular fatty acids were C14:0, C16:0 and C18:1. Based on phylogenetic analysis of 16S rRNA and recA gene sequences, we observed that the closest relatives of the isolate are moderately halophilic Bacillus species, with 16S rRNA gene sequence similarity ranging from 96.6 to 97.4% (Bacillus marisflavi, Bacillus aquimaris and Bacillus vietnamensis). Additionally, using genomic data it was determined that the type strain contains a total of nine rRNA operons with three slightly different sequences. On the basis of phenotypic and molecular properties, strain m4-4T represents a hovel species within the genus Bacillus, for which the name Bacillus coahuilensis sp. no,v. is proposed, with the type strain m4-4T (=NRRL B-41737T =CECT 7197T).
UR - http://www.scopus.com/inward/record.url?scp=44449086802&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.64959-0
DO - 10.1099/ijs.0.64959-0
M3 - Article
C2 - 18398195
AN - SCOPUS:44449086802
VL - 58
SP - 919
EP - 923
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 4
ER -